Type

Journal Article

Authors

Sinead M. Waters
Alan Kelly
Matthew S. McCabe
Paul Cormican
David A. Kenny
Kate Keogh

Subjects

Veterinary

Topics
growth rate cattle feed efficiency skeletal muscle transcriptional profiles holstein friesian gene expression energy production compensatory growth cg

Effect of Dietary Restriction and Subsequent Re-Alimentation on the Transcriptional Profile of Bovine Skeletal Muscle (2016)

Abstract Compensatory growth (CG), an accelerated growth phenomenon which occurs following aperiod of dietary restriction is exploited worldwide in animal production systems as a methodto lower feed costs. However the molecular mechanisms regulated CG expression remainto be elucidated fully. This study aimed to uncover the underlying biology regulating CG incattle, through an examination of skeletal muscle transcriptional profiles utilising next generationmRNA sequencing technology. Twenty Holstein Friesian bulls were fed either arestricted diet for 125 days, with a target growth rate of 0.6 kg/day (Period 1), followingwhich they were allowed feed ad libitum for a further 55 days (Period 2) or fed ad libitum forthe entirety of the trial. M. longissimus dorsi biopsies were harvested from all bulls on days120 and 15 of periods 1 and 2 respectively and RNAseq analysis was performed. During realimentationin Period 2, previously restricted animals displayed CG, growing at 1.8 timesthe rate of the ad libitum control animals. Compensating animals were also more feed efficientduring re-alimentation and compensated for 48% of their previous dietary restriction.1,430 and 940 genes were identified as significantly differentially expressed (BenjaminiHochberg adjusted P < 0.1) in periods 1 and 2 respectively. Additionally, 2,237 genes weredifferentially expressed in animals undergoing CG relative to dietary restriction. Dietaryrestriction in Period 1 was associated with altered expression of genes involved in lipidmetabolism and energy production. CG expression in Period 2 occurred in association withgreater expression of genes involved in cellular function and organisation. This study highlightssome of the molecular mechanisms regulating CG in cattle. Differentially expressedgenes identified are potential candidate genes for the identification of biomarkers for CGand feed efficiency, which may be incorporated into future breeding programmes
Collections Ireland -> Teagasc -> Animal & Bioscience

Full list of authors on original publication

Sinead M. Waters, Alan Kelly, Matthew S. McCabe, Paul Cormican, David A. Kenny, Kate Keogh

Experts in our system

1
Sinead M. Waters
Teagasc
Total Publications: 92
 
2
Alan K Kelly
Teagasc
Total Publications: 83
 
3
Matthew S. McCabe
Teagasc
Total Publications: 40
 
4
Paul Cormican
Teagasc
Total Publications: 44
 
5
D.A. Kenny
Teagasc
Total Publications: 138
 
6
Kate Keogh
Teagasc
Total Publications: 18