Journal Article


Colin Hill
R. Paul Ross
Paul D. Cotter
Rory O'Connor
Des Field



bacterial resistance penicillin v antibacterial activity growth curve antimicrobial resistance antimicrobial peptides gram positive bacteria methicillin resistant staphylococcus aureus staphylococci antimicrobial peptide nisin public health antibiotics lantibiotic bacteriocin biofilm

In Vitro Activities of Nisin and Nisin Derivatives Alone and In Combination with Antibiotics against Staphylococcus Biofilms (2016)

Abstract The development and spread of pathogenic bacteria that are resistant to the existingcatalog of antibiotics is a major public health threat. Biofilms are complex, sessilecommunities of bacteria embedded in an organic polymer matrix which serve to furtherenhance antimicrobial resistance. Consequently, novel compounds and innovativemethods are urgently required to arrest the proliferation of drug-resistant infections inboth nosocomial and community environments. Accordingly, it has been suggestedthat antimicrobial peptides could be used as novel natural inhibitors that can be usedin formulations with synergistically acting antibiotics. Nisin is a member of the lantibioticfamily of antimicrobial peptides that exhibit potent antibacterial activity against manyGram-positive bacteria. Recently we have used bioengineering strategies to enhancethe activity of nisin against several high profile targets, including multi-drug resistantclinical pathogens such as methicillin-resistant Staphylococcus aureus, vancomycinresistantenterococci, staphylococci, and streptococci associated with bovine mastitis.We have also identified nisin derivatives with an enhanced ability to impair biofilmformation and to reduce the density of established biofilms of methicillin resistantS. pseudintermedius. The present study was aimed at evaluating the potential ofnisin and nisin derivatives to increase the efficacy of conventional antibiotics andto assess the possibility of killing and/or eradicating biofilm-associated cells of avariety of staphylococcal targets. Growth curve-based comparisons established thatcombinations of derivatives nisin V C penicillin or nisin I4V C chloramphenicol hadan enhanced inhibitory effect against S. aureus SA113 and S. pseudintermediusDSM21284, respectively, compared to the equivalent nisin A C antibiotic combinationsor when each antimicrobial was administered alone. Furthermore, the metabolic activityof established biofilms treated with nisin V C chloramphenicol and nisin I4V Cchloramphenicol combinations revealed a significant decrease in S. aureus SA113 andS. pseudintermedius DSM21284 biofilm viability, respectively, compared to the nisin A Cantibiotic combinations as determined by the rapid colorimetric XTT assay. The resultsindicate that the activities of the nisin derivative and antibiotic combinations represent asignificant improvement over that of the wild-type nisin and antibiotic combination andmerit further investigation with a view to their use as anti-biofilm agents.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> Teagasc -> Food Biosciences
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Colin Hill, R. Paul Ross, Paul D. Cotter, Rory O'Connor, Des Field

Experts in our system

Colin Hill
University College Cork
Total Publications: 351
R Paul Ross
Total Publications: 441
Paul D. Cotter
Total Publications: 253
Des Field
Total Publications: 30