Type

Journal Article

Authors

David E. MacHugh
Eamonn Gormley
Stephen V Gordon
Kieran G Meade
Irene Martin
David A. Magee
Stephen D. Park
Karsten Hokamp
John A Browne
Cliona O'Farrelly
and 1 others

Subjects

Microbiology

Topics
genome wide transcriptional profiling systems analysis gene expression data animals immune suppression holstein friesian immunity innate genes society microbiology gene expression profiling genome science and technology leukocytes bovine tuberculosis host immune response agriculture fisheries food reverse transcriptase polymerase chain reaction female immunology immunology inflammation infection cattle peripheral blood leukocytes genetics mycobacterium bovis transcription genetic

Genome-wide transcriptional profiling of peripheral blood leukocytes from cattle infected with Mycobacterium bovis reveals suppression of host immune genes (2011)

Abstract Background: Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), a pathological infection with significant economic impact. Recent studies have highlighted the role of functional genomics to better understand the molecular mechanisms governing the host immune response to M. bovis infection. Furthermore, these studies may enable the identification of novel transcriptional markers of BTB that can augment current diagnostic tests and surveillance programmes. In the present study, we have analysed the transcriptome of peripheral blood leukocytes (PBL) from eight M. bovis-infected and eight control non-infected age-matched and sex-matched Holstein-Friesian cattle using the Affymetrix(R) GeneChip(R) Bovine Genome Array with 24,072 gene probe sets representing more than 23,000 gene transcripts. Results: Control and infected animals had similar mean white blood cell counts. However, the mean number of lymphocytes was significantly increased in the infected group relative to the control group (P = 0.001), while the mean number of monocytes was significantly decreased in the BTB group (P = 0.002). Hierarchical clustering analysis using gene expression data from all 5,388 detectable mRNA transcripts unambiguously partitioned the animals according to their disease status. In total, 2,960 gene transcripts were differentially expressed (DE) between the infected and control animal groups (adjusted P-value threshold [less than or equal to] 0.05); with the number of gene transcripts showing decreased relative expression (1,563) exceeding those displaying increased relative expression (1,397). Systems analysis using the Ingenuity(R) Systems Pathway Analysis (IPA) Knowledge Base revealed an over-representation of DE genes involved in the immune response functional category. More specifically, 64.5% of genes in the affects immune response subcategory displayed decreased relative expression levels in the infected animals compared to the control group. Conclusions: This study demonstrates that genome-wide transcriptional profiling of PBL can distinguish active M. bovis-infected animals from control non-infected animals. Furthermore, the results obtained support previous investigations demonstrating that mycobacterial infection is associated with host transcriptional suppression. These data support the use of transcriptomic technologies to enable the identification of robust, reliable transcriptional markers of active M. bovis infection.
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Full list of authors on original publication

David E. MacHugh, Eamonn Gormley, Stephen V Gordon, Kieran G Meade, Irene Martin, David A. Magee, Stephen D. Park, Karsten Hokamp, John A Browne, Cliona O'Farrelly and 1 others

Experts in our system

1
David E. MacHugh
University College Dublin
Total Publications: 82
 
2
E Gormley
University College Dublin
Total Publications: 68
 
3
Stephen V. Gordon
University College Dublin
Total Publications: 40
 
4
Kieran G Meade
Teagasc
Total Publications: 58
 
5
David A. Magee
University College Dublin
Total Publications: 47
 
6
Stephen D. E. Park
University College Dublin
Total Publications: 35
 
7
Karsten Hokamp
Trinity College Dublin
Total Publications: 47
 
8
John A Browne
University College Dublin
Total Publications: 73
 
9
Cliona O'Farrelly
Trinity College Dublin
Total Publications: 147