Type

Journal Article

Authors

Robert O'Connor
William Watson
Lorraine O'Driscoll
Paula Meleady
Paul Dowling
Michael Henry
Sweta Rani
Claire Corcoran
Amanda O'Neill
Maria Prencipe
and 1 others

Subjects

Biochemistry

Topics
prostate cancer docetaxel cancer cell lines response elements heat shock response cancer biochemistry cell lines proteins

The use of LC-MS to identify differentially expressed proteins in docetaxel-resistant prostate cancer cell lines (2012)

Abstract Docetaxel is a taxane-derived chemotherapy drug that has been approved for treatment of prostate cancer. While docetaxel is frequently used as a treatment for hormone-refractory prostate cancer, a subset of patients either do not respond to this treatment or those that do respond eventually become resistant to the drug over time. Resistance to docetaxel is complex and multi-factoral and further understanding of the cellular biochemistry underlying resistance is vital to improve treatment efficacy. To identify proteins altered in the resistant phenotype, three parental cell lines DU145, 22RV1 and PC-3, as well as their docetaxel resistant sub-lines, were subjected to quantitative label-free LC-MS proteomic profiling. A total of 189 significant (p < 0.05) protein abundance changes were identified in the DU145 resistant sub-lines, 254 in the 22RV1 sub-lines, and 51 and 72 in the 8 and 12 nM resistant PC-3 sub-lines, respectively. From these, 29 proteins demonstrated a significant (p < 0.05) fold change across two or more resistant variants. These included proteins indicative of an epithelial-to-mesenchemyl transition as well as altered heat shock response elements.
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Ireland -> Trinity College Dublin -> Pharmacy and Pharmaceutical Sciences
Ireland -> Maynooth University -> Type = Article
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Full list of authors on original publication

Robert O'Connor, William Watson, Lorraine O'Driscoll, Paula Meleady, Paul Dowling, Michael Henry, Sweta Rani, Claire Corcoran, Amanda O'Neill, Maria Prencipe and 1 others

Experts in our system

1
Robert O'Connor
Dublin City University
Total Publications: 74
 
2
William R Watson
Trinity College Dublin
 
3
Lorraine O'Driscoll
Trinity College Dublin
Total Publications: 164
 
4
Paula Meleady
Dublin City University
Total Publications: 115
 
5
Paul Dowling
Maynooth University
Total Publications: 82
 
6
Michael Henry
Dublin City University
Total Publications: 99
 
7
Sweta Rani
Trinity College Dublin
 
8
Claire Corcoran
Trinity College Dublin
 
9
Amanda J O'Neill
University College Dublin
Total Publications: 46
 
10
Maria Prencipe
University College Dublin
Total Publications: 19