Type

Journal Article

Authors

Norma O'Donovan
John Crown
Martin Clynes
Paula Meleady
Padraig Doolan
Michael Henry
Paul Dowling
Alex J. Eustace

Subjects

Biochemistry

Topics
cell lines phosphorylation treatment cells 2d dige melanoma dasatinib annexin a2

2D-DIGE analysis of phospho-enriched fractions from dasatinib-treated melanoma cell lines (2011)

Abstract Current therapeutic regimes for metastatic melanoma have failed to provide robust clinical responses. Dasatinib has shown anti-proliferative and anti-invasive effects in vitro; however, not all melanoma cells tested were sensitive to dasatinib. We used 2D-DIGE analysis of phospho-enriched fractions to identify phosphoproteins involved in regulating response to dasatinib in an isogenic pair of melanoma cell lines, one sensitive to dasatinib (WM-115) and the other resistant (WM-266-4). In WM-115 cells treated with dasatinib, 18 unique protein species with altered phosphorylation levels were detected. Dasatinib treatment of WM-266-4 cells resulted in phosphoprotein alterations to four unique protein species. Four phosphorylated forms of Annexin-A2 (ANXA2) were increased in WM-115 cells treated with dasatinib, whilst dasatinib treatment did not alter ANXA2 phosphoprotein levels in WM-266-4 cells. Immunoblotting confirmed that phosphorylation of ANXA2, on tyrosine residues, was increased in WM-115 cells treated with dasatinib. Subsequent knockdown of ANXA2 by siRNA significantly inhibited proliferation of WM-115 cells but did not significantly reduce proliferation of WM-266-4 cells. Therefore, ANXA2 plays a role in regulating proliferation in dasatinib-sensitive WM-115 cells and could potentially play a role in sensitivity to dasatinib in melanoma cells.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Norma O'Donovan, John Crown, Martin Clynes, Paula Meleady, Padraig Doolan, Michael Henry, Paul Dowling, Alex J. Eustace

Experts in our system

1
Norma O'Donovan
Dublin City University
Total Publications: 52
 
2
John Crown
Dublin City University
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Paula Meleady
Dublin City University
Total Publications: 95
 
5
Padraig Doolan
Dublin City University
Total Publications: 37
 
6
Michael Henry
Dublin City University
Total Publications: 78
 
7
Paul Dowling
Dublin City University
Total Publications: 79
 
8
Alex J Eustace
Royal College of Surgeons in Ireland
Total Publications: 23