Type

Journal Article

Authors

Tim Downing
Andrew T. Lloyd
Brenda Allan
Cliona O'Farrelly
Kieran G. Meade
Daniel Gerard Mary Bradley
Sarah Connell

Subjects

Veterinary

Topics
genome wide association bioinformatics immunology genetics genome wide association study immunity calmodulin intestinal homeostasis epithelial cell invasion gwas cadherin

Genome-Wide Association Analysis of Avian Resistance to Campylobacter jejuni Colonization Identifies Risk Locus Spanning the CDH13 Gene (2013)

Abstract The enteropathogen Campylobacter jejuni is a major worldwide health and economic burden, being one of the leading causes of bacterial gastroenteritis and commonly linked to postinfectious onset of autoimmune disease. Chickens are a major vector for human infection and even though variation in avian colonization level is heritable, no previous studies have identified regions of the genome associated with colonization resistance. We performed a genome-wide association study of resistance to C. jejuni colonization in the avian intestine by controlling for population structure, which revealed a risk locus with genome-wide significance spanning the T-cadherin (CDH13) gene. A second possible risk locus was also identified close to calmodulin (CALM1), a calcium-activated modulator of cadherin function. In addition, gene expression analysis of mRNA sequencing profiles revealed that the relative expression of the two genes is significantly associated with colonization resistance. Functional studies have previously demonstrated involvement of cadherins and calmodulin in C. jejuni intracellular invasion and colonization of human intestinal epithelial cells in vitro. Consistent with this finding, our analysis reveals that variation surrounding these genes is associated with avian colonization resistance in vivo and highlights their potential as possible targets for control of the bacterium in avian and human populations.
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Full list of authors on original publication

Tim Downing, Andrew T. Lloyd, Brenda Allan, Cliona O'Farrelly, Kieran G. Meade, Daniel Gerard Mary Bradley, Sarah Connell

Experts in our system

1
Tim Downing
Dublin City University
Total Publications: 25
 
2
Andrew T. Lloyd
Trinity College Dublin
Total Publications: 17
 
3
Cliona O'Farrelly
Trinity College Dublin
Total Publications: 147
 
4
Kieran G Meade
Teagasc
Total Publications: 58
 
5
Daniel Bradley
Trinity College Dublin
Total Publications: 84
 
6
Sarah Connell
Trinity College Dublin
Total Publications: 5