Type

Conference Proceedings

Authors

Fiona Regan
Jens Ducree
Richard O'Kennedy
Charles Nwankire
Caroline Murphy
Brendan Heery
Jenny Fitzgerald
Ivan Maguire

Subjects

Environment

Topics
ease of use chemistry fresh water aquatic monitoring sensitivity and specificity microcystin toxins analytical chemistry water quality monitoring

Development of an autonomous algal toxin analytical platform for aquatic monitoring (2016)

Abstract Cyclic peptide cyanobacterial toxins, in particular Microcystis aeruginosa, pose a serious health risk to humans and animals alike [1], [2]. Occurring mostly in fresh and brackish water, they have been identified to cause cancer promotion and liver damage [3]. Herein, we describe a portable, microfluidic-based system for in-situ detection of algal toxins in fresh water. The technology development presented here is a fully integrated and portable sample-to-answer centrifugal microfluidics-based system for the detection of toxic cyanobacteria – Microcystin-LR in fresh water. Our unique system employs highly-specific recombinant chicken anti-microcystin antibodies, prepared in-house, with a 3D-printed ‘LASER-photo¬diode’ fluorescent detection technique, also developed in-house. The system has high analytical specificity and sensitivity for detection of toxins below the regulatory limit with intra/inter day coefficient of variation of less than 20%. Dissolvable-film based valving technique was used for flow actuation and integration of multiple assays on the centrifugal cartridge. This new approach forms the basis of a cost efficient, USB-controlled water quality monitoring system. Technically, this integrated system consists of two components; a microfluidic disc (figure 1.A), the disc-holder fabricated and assembled from a 3D-printed casing, with electronic components housed in device. The 5-layered microfluidic disc consists of five reservoirs (figure 1.B), each with a separate venti-lation, aligned radially with inter-connected microchannels. A competitive immunoassay format is utilised to detect free toxin (figure 1.C). Sensitivity, reproducibility and ease-of-use are key features of this monitoring device. The ‘top-down’ optical detection system has been modified for improved detection sensitivity, as well as the elimination of external noise.
Collections Ireland -> Dublin City University -> Publication Type = Conference or Workshop Item
Ireland -> Dublin City University -> Subject = Physical Sciences: Analytical chemistry
Ireland -> Dublin City University -> Subject = Physical Sciences: Chemistry
Ireland -> Dublin City University -> Status = Unpublished
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Chemical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: Marine and Environmental Sensing Technology Hub (MESTECH)
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Physical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology

Full list of authors on original publication

Fiona Regan, Jens Ducree, Richard O'Kennedy, Charles Nwankire, Caroline Murphy, Brendan Heery, Jenny Fitzgerald, Ivan Maguire

Experts in our system

1
Fiona Regan
Dublin City University
Total Publications: 101
 
2
Jens Ducree
Dublin City University
Total Publications: 83
 
3
R O'Kennedy
Dublin City University
Total Publications: 197
 
4
Charles Nwankire
Dublin City University
Total Publications: 22
 
5
Caroline Murphy
Dublin City University
Total Publications: 12
 
6
Brendan Heery
Dublin City University
Total Publications: 31
 
7
Jenny Fitzgerald
Dublin City University
Total Publications: 18
 
8
Ivan Maguire
Dublin City University
Total Publications: 15