Type

Journal Article

Authors

Chris Maragos
Sarah De Saeger
Richard O'Kennedy
Mia Eeckhout
Yirong Guo
Kara Moran
Daniel McPartlin
Melanie Sanders

Subjects

Microbiology

Topics
surface plasmon resonance dust enzyme linked immunosorbent assay plasmon resonance spr screening assay matrix effects sample preparation interferometry

Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust. (2016)

Abstract A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 μg/kg, LOD wheat dust: 458 μg/kg).
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Chris Maragos, Sarah De Saeger, Richard O'Kennedy, Mia Eeckhout, Yirong Guo, Kara Moran, Daniel McPartlin, Melanie Sanders

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197