Type

Journal Article

Authors

Lourdes Basabe-Desmonts
Nigel J Kent
Antonio J Ricco
Dermot Kenny
Martin Somers
Niamh Gilmartin
Peter McCluskey
Bincy Jose

Subjects

Biochemistry

Topics
protein binding blood flow cardiovascular disease cvd surface protein microfluidic device platelet aggregation protein protein interface cell adhesion

Self-Powered Microfluidic Device for Rapid Assay of Antiplatelet Drugs. (2016)

Abstract We report the development of a microfluidic device for the rapid assay in whole blood of interfacial platelet-protein interactions indicative of the efficacy of antiplatelet drugs, for example, aspirin and Plavix, two of the world's most widely used drugs, in patients with cardiovascular disease (CVD). Because platelet adhesion to surface-confined protein matrices is an interfacial phenomenon modulated by fluid shear rates at the blood/protein interface, and because such binding is a better indicator of platelet function than platelet self-aggregation, we designed, fabricated, and characterized the performance of a family of disposable, self-powered microfluidic chips with well-defined flow and interfacial shear rates suitable for small blood volumes (≤200 μL). This work demonstrates that accurate quantification of cell adhesion to protein matrices, an important interfacial biological phenomenon, can be used as a powerful diagnostic tool in those with CVD, the world's leading cause of death. To enable such measurements, we developed a simple technique to fabricate single-use self-powered chips incorporating shear control (SpearChips). These parallel-plate flow devices integrate on-chip vacuum-driven blood flow, using a predegassed elastomer component to obviate active pumping, with microcontact-printed arrays of 6-μm-diameter fluorescently labeled fibrinogen dots on a cyclic olefin polymer base plate as a means to quantitatively count platelet-protein binding events. The use of SpearChips to assess in whole blood samples the effects of GPIIb/IIIa and P2Y12 inhibitors, two important classes of "antiplatelet" drugs, is reported.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Lourdes Basabe-Desmonts, Nigel J Kent, Antonio J Ricco, Dermot Kenny, Martin Somers, Niamh Gilmartin, Peter McCluskey, Bincy Jose

Experts in our system

1
Lourdes Basabe-Desmonts
Dublin City University
Total Publications: 15
 
2
Nigel J Kent
Dublin City University
Total Publications: 18
 
3
Antonio J. Ricco
Dublin City University
Total Publications: 24
 
4
Dermot Kenny
Royal College of Surgeons in Ireland
 
5
Peter McCluskey
Dublin City University
Total Publications: 17