Journal Article


Robert Foronjy
Matthias Salathe
Astrid Grosche
Edward Eden
Neville Cummins
Bakr Jundi
Abdoulaye Jules Dabo
Michael Campos
Nathalie Baumlin
Patrick Geraghty
and 1 others



human cells interleukin 6 epithelial cells cystic fibrosis lung disease mice cytokine production il 6

Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner. (2015)

Abstract The use of electronic (e)-cigarettes is increasing rapidly, but their lung health effects are not established. Clinical studies examining the potential long-term impact of e-cigarette use on lung health will take decades. To address this gap in knowledge, this study investigated the effects of exposure to aerosolised nicotine-free and nicotine-containing e-cigarette fluid on mouse lungs and normal human airway epithelial cells. Mice were exposed to aerosolised phosphate-buffered saline, nicotine-free or nicotine-containing e-cigarette solution, 1-hour daily for 4 months. Normal human bronchial epithelial (NHBE) cells cultured at an air-liquid interface were exposed to e-cigarette vapours or nicotine solutions using a Vitrocell smoke exposure robot. Inhalation of nicotine-containing e-cigarettes increased airway hyper-reactivity, distal airspace enlargement, mucin production, cytokine and protease expression. Exposure to nicotine-free e-cigarettes did not affect these lung parameters. NHBE cells exposed to nicotine-containing e-cigarette vapour showed impaired ciliary beat frequency, airway surface liquid volume, cystic fibrosis transmembrane regulator and ATP-stimulated K+ ion conductance and decreased expression of FOXJ1 and KCNMA1. Exposure of NHBE cells to nicotine for 5 days increased interleukin (IL)-6 and IL-8 secretion. Exposure to inhaled nicotine-containing e-cigarette fluids triggered effects normally associated with the development of COPD including cytokine expression, airway hyper-reactivity and lung tissue destruction. These effects were nicotine-dependent both in the mouse lung and in human airway cells, suggesting that inhaled nicotine contributes to airway and lung disease in addition to its addictive properties. Thus, these findings highlight the potential dangers of nicotine inhalation during e-cigarette use.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Robert Foronjy, Matthias Salathe, Astrid Grosche, Edward Eden, Neville Cummins, Bakr Jundi, Abdoulaye Jules Dabo, Michael Campos, Nathalie Baumlin, Patrick Geraghty and 1 others

Experts in our system

Patrick Geraghty
Royal College of Surgeons in Ireland
Total Publications: 13