Type

Journal Article

Authors

Fergal J O'Brien
Garry P Duffy
Caroline M Curtin
Irene Mencía Castaño

Subjects

Biochemistry

Topics
tissue engineering tissue engineering scaffolds collagen scaffolds mesenchymal stem cell bone tissue engineering stem cells non viral novel the

Next generation bone tissue engineering: non-viral miR-133a inhibition using collagen-nanohydroxyapatite scaffolds rapidly enhances osteogenesis. (2015)

Abstract Bone grafts are the second most transplanted materials worldwide at a global cost to healthcare systems valued over $30 billion every year. The influence of microRNAs in the regenerative capacity of stem cells offers vast therapeutic potential towards bone grafting; however their efficient delivery to the target site remains a major challenge. This study describes how the functionalisation of porous collagen-nanohydroxyapatite (nHA) scaffolds with miR-133a inhibiting complexes, delivered using non-viral nHA particles, enhanced human mesenchymal stem cell-mediated osteogenesis through the novel focus on a key activator of osteogenesis, Runx2. This study showed enhanced Runx2 and osteocalcin expression, as well as increased alkaline phosphatase activity and calcium deposition, thus demonstrating a further enhanced therapeutic potential of a biomaterial previously optimised for bone repair applications. The promising features of this platform offer potential for a myriad of applications beyond bone repair and tissue engineering, thus presenting a new paradigm for microRNA-based therapeutics.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Garry P Duffy, Caroline M Curtin, Irene Mencía Castaño

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
2
Garry P Duffy
Royal College of Surgeons in Ireland
Total Publications: 59
 
3
Caroline M Curtin
Royal College of Surgeons in Ireland
Total Publications: 22
 
4
Irene Mencía Castaño
Royal College of Surgeons in Ireland
Total Publications: 8