Type

Journal Article

Authors

Dominik Hartl
N Gerry McElvaney
Matthias Griese
Michelle A Murray
Ute Graepler-Mainka
Joachim Riethmüller
Regina Ensenauer
Ines Mack
Sina Berenbrinker
Anjai Ralhan
and 8 others

Subjects

Microbiology

Topics
lung disease bronchoalveolar lavage fluid deficiency patients cystic fibrosis chitinase activation candida albicans

Chitinase activation in patients with fungus-associated cystic fibrosis lung disease. (2015)

Abstract Chitinases have recently gained attention in the field of pulmonary diseases, particularly in asthma and chronic obstructive pulmonary disease, but their potential role in patients with cystic fibrosis (CF)-associated lung disease remains unclear. The aim of this study was to assess chitinase activity systemically and in the airways of patients with CF and asthma compared with healthy subjects. Additionally, we assessed factors that regulate chitinase activity within the lungs of patients with CF. Chitinase activities were quantified in serum and bronchoalveolar lavage fluid from patients with CF, asthmatic patients, and healthy control subjects. Mechanistically, the role of CF airway proteases and genetic chitinase deficiency was assessed. Chitinase activity was systemically increased in patients with CF compared with that in healthy control subjects and asthmatic patients. Further stratification showed that chitinase activity was enhanced in patients with CF colonized with Candida albicans compared with that in noncolonized patients. CF proteases degraded chitinases in the airway microenvironment of patients with CF. Genetic chitinase deficiency was associated with C albicans colonization in patients with CF. Patients with CF have enhanced chitinase activation associated with C albicans colonization. Therefore chitinases might represent a novel biomarker and therapeutic target for CF-associated fungal disease.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Dominik Hartl, N Gerry McElvaney, Matthias Griese, Michelle A Murray, Ute Graepler-Mainka, Joachim Riethmüller, Regina Ensenauer, Ines Mack, Sina Berenbrinker, Anjai Ralhan and 8 others

Experts in our system

1
Dominik Hartl
Royal College of Surgeons in Ireland
Total Publications: 3
 
2
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194