Type

Journal Article

Authors

Colin Hill
R. Paul Ross
Fabien J. Cousin
Charles M A P Franz
Horst Neve
Ronan Strain
Marion Dalmasso

Subjects

Microbiology

Topics
escherichia coli infections phages therapy bacterial biofilms bacteriophages gene prediction antibiotics escherichia coli gut show biofilm formation human comparative genomics viral genomics bird genomics anti microbial

Three New Escherichia coli Phages from the Human Gut Show Promising Potential for Phage Therapy. (2016)

Abstract With the emergence of multi-drug resistant bacteria the use of bacteriophages (phages) is gaining renewed interest as promising anti-microbial agents. The aim of this study was to isolate and characterize phages from human fecal samples. Three new coliphages, ɸAPCEc01, ɸAPCEc02 and ɸAPCEc03, were isolated. Their phenotypic and genomic characteristics, and lytic activity against biofilm, and in combination with ciprofloxacin, were investigated. All three phages reduced the growth of E. coli strain DPC6051 at multiplicity of infection (MOI) between 10-3 and 105. A cocktail of all three phages completely inhibited the growth of E. coli. The phage cocktail also reduced biofilm formation and prevented the emergence of phage-resistant mutants which occurred with single phage. When combined with ciprofloxacin, phage alone or in cocktail inhibited the growth of E. coli and prevented the emergence of resistant mutants. These three new phages are promising biocontrol agents for E. coli infections.
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Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles

Full list of authors on original publication

Colin Hill, R. Paul Ross, Fabien J. Cousin, Charles M A P Franz, Horst Neve, Ronan Strain, Marion Dalmasso

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Fabien J. Cousin
University College Cork
Total Publications: 3
 
4
Charles M. Franz
University College Cork
Total Publications: 6
 
5
Horst Neve
Teagasc
 
6
Marion Dalmasso
Teagasc
Total Publications: 13