Type

Journal Article

Authors

Douwe van Sinderen
Christian Cambillau
Fabio Dal Bello
Jean-Paul Noben
Jennifer Mahony
Eoghan Casey
Silvia Spinelli
Stéphanie Blangy
Evgeny Vinogradov
Irina Sadovskaya
and 1 others

Subjects

Microbiology

Topics
gram positive bacteria cell wall lactobacillus complex carbohydrates lactococcus lactis bacteriophage processes surface complex

The Baseplate of Lactobacillus delbrueckii Bacteriophage Ld17 Harbors a Glycerophosphodiesterase. (2016)

Abstract Glycerophosphodiester phosphodiesterases (GDPDs; EC 3.1.4.46) typically hydrolyze glycerophosphodiesters to sn-glycerol 3-phosphate (Gro3P) and their corresponding alcohol during patho/physiological processes in bacteria and eukaryotes. GDPD(-like) domains were identified in the structural particle of bacterial viruses (bacteriophages) specifically infecting Gram-positive bacteria. The GDPD of phage 17 (Ld17; GDPDLd17), representative of the group b Lactobacillus delbrueckii subsp. bulgaricus (Ldb)-infecting bacteriophages, was shown to hydrolyze, besides the simple glycerophosphodiester, two complex surface-associated carbohydrates of the Ldb17 cell envelope: the Gro3P decoration of the major surface polysaccharide d-galactan and the oligo(glycerol phosphate) backbone of the partially glycosylated cell wall teichoic acid, a minor Ldb17 cell envelope component. Degradation of cell wall teichoic acid occurs according to an exolytic mechanism, and Gro3P substitution is presumed to be inhibitory for GDPDLd17 activity. The presence of the GDPDLd17 homotrimer in the viral baseplate structure involved in phage-host interaction together with the dependence of native GDPD activity, adsorption, and efficiency of plating of Ca(2+) ions supports a role for GDPDLd17 activity during phage adsorption and/or phage genome injection. In contrast to GDPDLd17, we could not identify any enzymatic activity for the GDPD-like domain in the neck passage structure of phage 340, a 936-type Lactococcus lactis subsp. lactis bacteriophage.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Christian Cambillau, Fabio Dal Bello, Jean-Paul Noben, Jennifer Mahony, Eoghan Casey, Silvia Spinelli, Stéphanie Blangy, Evgeny Vinogradov, Irina Sadovskaya and 1 others

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Christian Cambillau
University College Cork
Total Publications: 18
 
3
Fabio Dal Bello
University College Cork
Total Publications: 7
 
4
Jean-Paul Noben
University College Cork
Total Publications: 13
 
5
Jennifer Mahony
University College Cork
Total Publications: 87
 
6
Eoghan Casey
University College Cork
Total Publications: 9
 
7
Silvia Spinelli
University College Cork
Total Publications: 6
 
8
Stéphanie Blangy
University College Cork
Total Publications: 4
 
9
Evgeny Vinogradov
University College Cork
 
10
Irina Sadovskaya
University College Cork
Total Publications: 10