Type

Journal Article

Authors

Ulla G Knaus
Billy Bourke
Dmitri Papkovsky
Rosanne Hertzberger
Lorraine Brennan
Marie-Helene Paclet
Alexander Zhdanov
Malgorzata Kubica
Gabriella Aviello
Gratiela Pircalabioru

Subjects

Biochemistry

Topics
mutualism environmental factors mice infection nadph oxidase bacterial strains gut reactive oxygen species

Defensive Mutualism Rescues NADPH Oxidase Inactivation in Gut Infection. (2015)

Abstract NOX/DUOX family of NADPH oxidases are expressed in diverse tissues and are the primary enzymes for the generation of reactive oxygen species (ROS). The intestinal epithelium expresses NOX1, NOX4, and DUOX2, whose functions are not well understood. To address this, we generated mice with complete or epithelium-restricted deficiency in the obligatory NOX dimerization partner Cyba (p22(phox)). We discovered that NOX1 regulates DUOX2 expression in the intestinal epithelium, which magnified the epithelial ROS-deficiency. Unexpectedly, epithelial deficiency of Cyba resulted in protection from C. rodentium and L. monocytogenes infection. Microbiota analysis linked epithelial Cyba deficiency to an enrichment of H2O2-producing bacterial strains in the gut. In particular, elevated levels of lactobacilli physically displaced and attenuated C. rodentium virulence by H2O2-mediated suppression of the virulence-associated LEE pathogenicity island. This transmissible compensatory adaptation relied on environmental factors, an important consideration for prevention and therapy of enteric disease.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Ulla G Knaus, Billy Bourke, Dmitri Papkovsky, Rosanne Hertzberger, Lorraine Brennan, Marie-Helene Paclet, Alexander Zhdanov, Malgorzata Kubica, Gabriella Aviello, Gratiela Pircalabioru

Experts in our system

1
Ulla G Knaus
University College Dublin
Total Publications: 24
 
2
Billy Bourke
University College Dublin
Total Publications: 37
 
3
Dmitri B Papkovsky
University College Cork
Total Publications: 89
 
4
Lorraine Brennan
University College Dublin
Total Publications: 166
 
5
Alexander V Zhdanov
University College Cork
Total Publications: 37