Type

Journal Article

Authors

Douwe van Sinderen
Christian Cambillau
Francois P Douillard
Silvia Spinelli
Barry Collins
Stephen R Stockdale
Jennifer Mahony

Subjects

Biochemistry

Topics
sequence homology membrane protein bacillus subtilis assembly functional analysis phage lactococcus lactis cell wall

Lactococcus lactis phage TP901-1 as a model for Siphoviridae virion assembly. (2015)

Abstract Phages infecting Lactococcus lactis pose a serious threat to the dairy fermentation sector. Consequently, they are among the most thoroughly characterized Gram positive-infecting phages. The majority of lactococcal phages belong to the tailed family of phages named the Siphoviridae. The coliphage lambda and the Bacillus subtilis phage SPP1 have been the predominant comparators for emerging siphophages both genomically and structurally and both phages recognize a membrane protein receptor. In contrast, the lactococcal P335 group phage TP901-1 attaches to cell wall surface polysaccharides. It is a typical "lambdoid" siphophage possessing a long non-contractile tail and a genomic architecture reminiscent of lambda and SPP1 despite low or undetectable sequence homology in many of its encoded products, especially those involved in host recognition. A functional analysis of the structural components of TP901-1 was undertaken based on the characterization of a series of mutants in the region encoding the capsid and tail morphogenetic elements. Through this analysis, it was possible to deduce that, despite the lack of sequence homology, the overall genomic architecture of Siphoviridae phages typified by functional synteny is conserved. Furthermore, a model of the TP901-1 assembly pathway was developed with potential implications for many tailed phages.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Christian Cambillau, Francois P Douillard, Silvia Spinelli, Barry Collins, Stephen R Stockdale, Jennifer Mahony

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Christian Cambillau
University College Cork
Total Publications: 18
 
3
Silvia Spinelli
University College Cork
Total Publications: 6
 
4
Barry Collins
University College Cork
Total Publications: 11
 
5
Stephen R. Stockdale
University College Cork
Total Publications: 15
 
6
Jennifer Mahony
University College Cork
Total Publications: 87