Type

Journal Article

Authors

C G M Gahan
S A Joyce
C Hill
M Kinsella
J A Nolan

Subjects

Chemistry

Topics
impact analysis cholesterol biosynthesis enterococcus faecium liquid chromatography mass spectrometry mass spectrometry listeria monocytogenes effective concentration staphylococcus aureus

Analysis of the Impact of Rosuvastatin on Bacterial Mevalonate Production Using a UPLC-Mass Spectrometry Approach. (2015)

Abstract Statins are widely prescribed cholesterol-lowering medications and act through inhibition of the human enzyme 3-methylglutaryl coenzyme A reductase (HMG-R) which produces mevalonate (MVAL), a key substrate for cholesterol biosynthesis. Some important microbial species also express an isoform of HMG-R; however, the nature of the interaction between statins and bacteria is currently unclear and studies would benefit from protocols to quantify MVAL in complex microbial environments. The objective of this study was to develop a protocol for the analytical quantification of MVAL in bacterial systems and to utilise this approach to analyse the effects of Rosuvastatin (RSV) on bacterial MVAL formation. To determine the effective concentration range of RSV, we examined the dose-dependent inhibition of growth in the HMG-R(+) bacterial pathogens Listeria monocytogenes, Staphylococcus aureus and Enterococcus faecium at various concentrations of pure RSV. Growth inhibition generally correlated with a reduction in bacterial MVAL levels, particularly in culture supernatants at high RSV concentrations, as determined using our ultra-performance liquid chromatography mass spectrometry protocol. This work therefore outlines a refined protocol for the analysis of MVAL in microbial cultures and provides evidence for statin-mediated inhibition of bacterial HMG-R. Furthermore, we show that MVAL is readily transported and secreted from bacterial cells into the growth media.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

C G M Gahan, S A Joyce, C Hill, M Kinsella, J A Nolan

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Susan A. Joyce
University College Cork
Total Publications: 42
 
3
Colin Hill
University College Cork
Total Publications: 351