Type

Journal Article

Authors

Michael D Gilchrist
Eoin Casey
Nan Zhang
Brian J Rodriguez
Emmanuel G Reynaud
Peter Flood
Olivier Habimana
Seong Ying Choi

Subjects

Chemistry

Topics
bacterial adhesion pseudomonas fluorescens cell adhesion atomic force microscopy afm micro injection moulding microfluidic devices surface roughness adhesion effects

Material- and feature-dependent effects on cell adhesion to micro injection moulded medical polymers. (2015)

Abstract Two polymers, polymethylmethacrylate (PMMA) and cyclic olefin copolymer (COC), containing a range of nano- to micron- roughness surfaces (Ra 0.01, 0.1, 0.4, 1.0, 2.0, 3.2 and 5.0μm) were fabricated using electrical discharge machining (EDM) and replicated using micro injection moulding (μIM). Polymer samples were characterized using optical profilometry, atomic force microscopy (AFM) and water surface contact angle. Cell adhesion tests were carried out using bacterial Pseudomonas fluorescens and mammalian Madin-Darby Canine Kidney (MDCK) cells to determine the effect of surface hydrophobicity, surface roughness and stiffness. It is found that there are features which gave insignificant differences (feature-dependent effect) in cell adhesion, albeit a significant difference in the physicochemical properties (material-dependent effect) of substrata. In bacterial cell adhesion, the strongest feature-dependence is found at Ra 0.4μm surfaces, with material-dependent effects strongest at Ra 0.01μm. Ra 0.1μm surfaces exhibited strongest feature-dependent effects and Ra 5.0μm has strongest material-dependent effects on mammalian cell adhesion. Bacterial cell adhesion is found to be favourable to hydrophobic surfaces (COC), with the lowest adhesion at Ra 0.4μm for both materials. Mammalian cell adhesion is lowest in Ra 0.1μm and highest in Ra 1.0μm, and generally favours hydrophilic surfaces (PMMA). These findings can be used as a basis for developing medical implants or microfluidic devices using micro injection moulding for diagnostic purposes, by tuning the cell adhesion on different areas containing different surface roughnesses on the diagnostic microfluidic devices or medical implants.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Michael D Gilchrist, Eoin Casey, Nan Zhang, Brian J Rodriguez, Emmanuel G Reynaud, Peter Flood, Olivier Habimana, Seong Ying Choi

Experts in our system

1
M. D. Gilchrist
University College Dublin
Total Publications: 172
 
2
Eoin Casey
University College Dublin
Total Publications: 67
 
3
Nan Zhang
University College Dublin
Total Publications: 19
 
4
Brian J. Rodriguez
University College Dublin
Total Publications: 96
 
5
Olivier Habimana
University College Dublin
Total Publications: 22