Journal Article


Grace Mulcahy
Joseph Cassidy
Peter Diggle
Diana Williams
Robin J Flynn
Michael Welsh
Jim McNair
Alfonso Blanco
Jack O'Sullivan
Laura Garza-Cuartero



inflammatory cytokines bystander effect host pathogen interactions mycobacterium bovis pro inflammatory response immune response fasciola hepatica diagnostic tests

Fasciola hepatica Infection Reduces Mycobacterium bovis burden and Mycobacterial Uptake and Suppresses the Pro-inflammatory Response. (2015)

Abstract Bovine tuberculosis (BTB), caused by Mycobacterium bovis, has an annual incidence in cattle of 0.5% in the Republic of Ireland, and 4.7% in the UK, despite long-standing eradication programmes being in place. Failure to achieve complete eradication is multifactorial but the limitations of diagnostic tests are significant complicating factors. Previously, we have demonstrated that Fasciola hepatica infection, highly prevalent in these areas, induced reduced sensitivity of the standard diagnostic tests for BTB in animals co-infected with F. hepatica and M. bovis. This was accompanied by a reduced M. bovis specific Th1-immune response. We hypothesized that these changes in co-infected animals would be accompanied by enhanced growth of M. bovis. However, we show here that mycobacterial burden in cattle is reduced in animals co-infected with F. hepatica. Furthermore, we demonstrate a lower mycobacterial recovery and uptake in blood monocyte derived macrophages (MDM) from F. hepatica infected cattle which is associated with suppression of pro-inflammatory cytokines and a switch to alternative activation of macrophages. However, the cell surface expression of TLR2 and CD14 in MDM from F. hepatica infected cattle is increased. These findings reflecting the bystander effect of helminth-induced downregulation of pro-inflammatory responses provide insights to understand host-pathogen interactions in co-infection. This article is protected by copyright. All rights reserved.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Grace Mulcahy, Joseph Cassidy, Peter Diggle, Diana Williams, Robin J Flynn, Michael Welsh, Jim McNair, Alfonso Blanco, Jack O'Sullivan, Laura Garza-Cuartero

Experts in our system

G Mulcahy
University College Dublin
Total Publications: 91
Joseph P. Cassidy
University College Dublin
Total Publications: 55
Michael D Welsh
Total Publications: 11
Jack O'Sullivan
University College Dublin
Total Publications: 3
Laura Garza-Cuartero
University College Dublin
Total Publications: 6