Journal Article


Fiona C McGillicuddy
Helen M Roche
Muredach P Reilly
Margarita de la Llera Moya
Lorraine Brennan
Eileen Gibney
Mike Gibney
Miriam Ryan
Daniel Jones
Claire Lyons
and 6 others



transport acute phase proteins insulin resistance hepatic inflammation composition cholesterol hdl diet high fat h 3

High Density Lipoprotein Proteomic Composition, and Not Efflux Capacity, Reflects Differential Modulation of Reverse Cholesterol Transport by Saturated and Monounsaturated Fat Diets. (2015)

Abstract -Acute inflammation impairs Reverse Cholesterol Transport (RCT) and reduces high-density lipoprotein (HDL) functionin vivo This study hypothesized that obesity-induced inflammation impedes RCT and alters HDL composition, and investigated if dietary replacement of saturated (SFA) for monounsaturated (MUFA) fat modulates RCT. -Macrophage-to-feces RCT, HDL efflux capacity and HDL proteomic profiling was determined in C57BL/6j mice following 24 weeks on SFA or MUFA-enriched high fat diets (HFD) or low-fat diet (LFD). The impact of dietary SFA consumption and insulin resistance on HDL efflux function was also assessed in humans. Both HFDs increased plasma(3)H-cholesterol counts during RCTin vivoand ABCA1-independent efflux to plasmaex vivo, effects which were attributable to elevated HDL-cholesterol (C). By contrast ABCA1-dependent efflux was reduced after both HFDs, an effect that was also observed with insulin resistance and high SFA-consumption in humans. SFA-HFD impaired liver-to-feces RCT, increased hepatic inflammation and reduced ABCG5/8 and ABCB11 transporter expression compared to LFD, while liver-to-feces RCT was preserved after MUFA-HFD. HDL particles were enriched with acute-phase proteins (serum amyloid A, haptoglobin and hemopexin) and depleted of paraoxonase-1 after SFA-HFD compared to MUFA-HFD. -Ex vivoefflux assays validated increased macrophage-to-plasma RCTin vivoafter both HFDs but failed to capture differential modulation of hepatic cholesterol trafficking. By contrast proteomics revealed association of hepatic-derived inflammatory proteins on HDL after SFA-HFD compared to MUFA-HFD which reflected differential hepatic cholesterol trafficking between groups. Acute-phase protein levels on HDL may serve as novel biomarkers of impaired liver-to-feces RCTin vivo.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Fiona C McGillicuddy, Helen M Roche, Muredach P Reilly, Margarita de la Llera Moya, Lorraine Brennan, Eileen Gibney, Mike Gibney, Miriam Ryan, Daniel Jones, Claire Lyons and 6 others

Experts in our system

Fiona C McGillicuddy
University College Dublin
Total Publications: 19
Helen M. Roche
University College Dublin
Total Publications: 105
Lorraine Brennan
University College Dublin
Total Publications: 166
Eileen R Gibney
University College Dublin
Total Publications: 98
Miriam Ryan
University College Dublin
Total Publications: 34
Claire L Lyons
University College Dublin
Total Publications: 8