Type

Journal Article

Authors

Conor Buckley
Tatiana Vinardell
Daniel Kelly
S D Thorpe

Subjects

Biochemistry

Topics
chondrocytes tgf 3 cell separation physiology bioengineering sepharose elastic modulus patella animals mesenchymal stromal cells structure activity relationship osteogenesis glycosaminoglycans mechanical properties multipotent stem cells cells cultured cartilage infrapatellar fat pad next generation medical devices mesenchymal stem cells metabolism dna cytology sus scrofa collagen adipose tissue bone marrow cells hydrogel tissue engineering

Composition-function relations of cartilaginous tissues engineered from chondrocytes and mesenchymal stem cells isolated from bone marrow and infrapatellar fat pad (2010)

Abstract The objective of this study was to determine the functional properties of cartilaginous tissues generated by porcine MSCs isolated from different tissue sources, and to compare these properties to those derived from chondrocytes (CC). MSCs were isolated from bone marrow (BM) and infrapatellar fat pad (FP), while CC were harvested from the articular surface of the femoro-patellar joint. Culture-expanded CC and MSCs were encapsulated in agarose hydrogels and cultured in the presence of TGF-?3. Samples were analysed biomechanically, biochemically and histologically at day 0, day 21 and day 42. After 42 days in free swelling culture, mean GAG content was 1.50 % w/w in CC seeded constructs, compared to 0.95 % w/w in FP and 0.43 % w/w in BM seeded constructs. Total collagen accumulation was highest in FP constructs. DNA content increased with time for all the groups. The mechanical functionality of cartilaginous tissues engineered using CCs was superior to that generated from either source of MSCs. Differences were also observed in the spatial distribution of matrix components in tissues engineered using CC and MSCs, which appears to have a strong influence on the apparent mechanical properties of the constructs. Therefore while functional cartilaginous tissues can be engineered using MSCs isolated from different sources, the spatial composition of these tissues is unlike that generated using chondrocytes, suggesting that MSCs and chondrocytes respond differently to the regulatory factors present within developing cartilaginous constructs.
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Full list of authors on original publication

Conor Buckley, Tatiana Vinardell, Daniel Kelly, S D Thorpe

Experts in our system

1
Conor T Buckley
Trinity College Dublin
Total Publications: 85
 
2
Tatiana Vinardell
Trinity College Dublin
Total Publications: 27
 
3
Daniel Kelly
Trinity College Dublin
Total Publications: 168
 
4
Stephen D Thorpe
Trinity College Dublin
Total Publications: 18