Type

Journal Article

Authors

R. Paul Ross
Colin Hill
Mary C Rea
Beatriz Gómez-Sala
Orla O'Sullivan
Paula M. O'Connor
Fergus W. J. Collins

Subjects

Microbiology

Topics
fermented foods lactobacillus antimicrobial activity human microbiota lactobacillus acidophilus bacteria genome gene antimicrobials gram positive bacteria lactic acid bacteria

Bacteriocin Gene-Trait matching across the complete Lactobacillus Pan-genome (2017)

Abstract Lactobacilli constitute a large genus of Gram-positive lactic acid bacteria which have widespread roles ranging from gut commensals to starters in fermented foods. A combination of in silico and laboratory-based screening allowed us to determine the overall bacteriocin producing potential of representative strains of each species of the genus. The genomes of 175 lactobacilli and 38 associated species were screened for the presence of antimicrobial producing genes and combined with screening for antimicrobial activity against a range of indicators. There also appears to be a link between the strains’ environment and bacteriocin production, with those from the animal and human microbiota encoding over twice as many bacteriocins as those from other sources. Five novel bacteriocins were identified belonging to differing bacteriocin classes, including two-peptide bacteriocins (muricidin and acidocin X) and circular bacteriocins (paracyclicin). In addition, there was a clear clustering of helveticin type bacteriolysins in the Lactobacillus acidophilus group of species. This combined in silico and in vitro approach to screening has demonstrated the true diversity and complexity of bacteriocins across the genus. It also highlights their biological importance in terms of communication and competition between closely related strains in diverse complex microbial environments.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

R. Paul Ross, Colin Hill, Mary C Rea, Beatriz Gómez-Sala, Orla O'Sullivan, Paula M. O'Connor, Fergus W. J. Collins

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Mary C. Rea
Teagasc
Total Publications: 68
 
4
Beatriz Gómez-Sala
University College Cork
Total Publications: 3
 
5
Orla O'Sullivan
Teagasc
Total Publications: 92
 
6
Paula M. O'Connor
Teagasc
Total Publications: 85