Type

Journal Article

Authors

R. Paul Ross
Colin Hill
Paul D. Cotter
Mary C Rea
Paula M. O'Connor
Beatriz Mesa-Pereira

Subjects

Microbiology

Topics
class controlled protein engineering bactofencin bacteriocins pediocin pa 1 class iia bactofencin a class iid escherichia coli bacteriocin operons protein expression e coli genetic tools

Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli (2017)

Abstract The bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter protein. Cloning of these operons in E. coli TunerTM (DE3) on a pETcoco-2 derived vector resulted in successful secretion of both bacteriocins. A corresponding approach, involving the construction of vectors containing different combinations of these genes, revealed that the structural and the transporter genes alone are sufficient to permit heterologous production and secretion in this host. Even though the accessory protein, usually associated with optimal disulfide bond formation, was not required for bacteriocin synthesis, its presence did result in greater pediocin PA-1 production. The simplicity of the system and the fact that the associated bacteriocins could be recovered from the extracellular medium provides an opportunity to facilitate protein engineering and the overproduction of biologically-active bacteriocins at industrial scale. Additionally, this system could enable the characterization of new bacteriocin operons where genetic tools are not available for the native producers.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

R. Paul Ross, Colin Hill, Paul D. Cotter, Mary C Rea, Paula M. O'Connor, Beatriz Mesa-Pereira

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Mary C. Rea
Teagasc
Total Publications: 68
 
5
Paula M. O'Connor
Teagasc
Total Publications: 85