Type

Journal Article

Authors

Douwe van Sinderen
Christian Cambillau
Marine Feyereisen
Silvia Spinelli
Stephen Stockdale
Mona Alqarni
Jennifer Mahony

Subjects

Veterinary

Topics
phage chaperone formation membrane protein infection measure genes receptor c 60 structural bacteriophage lambda tail 1 protein dissection host dna baseplate length determination lactis

Functional and structural dissection of the tape measure protein of lactococcal phage TP901-1 (2016)

Abstract The tail tape measure protein (TMP) of tailed bacteriophages (also called phages) dictates the tail length and facilitates DNA transit to the cell cytoplasm during infection. Here, a thorough mutational analysis of the TMP from lactococcal phage TP901-1 (TMPTP901-1) was undertaken. We generated 56 mutants aimed at defining TMPTP901-1 domains that are essential for tail assembly and successful infection. Through analysis of the derived mutants, we determined that TP901-1 infectivity requires the N-terminal 154 aa residues, the C-terminal 60 residues and the first predicted hydrophobic region of TMPTP901-1 as a minimum. Furthermore, the role of TMPTP901-1 in tail length determination was visualized by electron microscopic imaging of TMP-deletion mutants. The inverse linear correlation between the extent of TMPTP901-1-encoding gene deletions and tail length of the corresponding virion provides an estimate of TMPTP901-1 regions interacting with the connector or involved in initiator complex formation. This study represents the most thorough characterisation of a TMP from a Gram-positive host-infecting phage and provides essential advances to understanding its role in virion assembly, morphology and infection.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Christian Cambillau, Marine Feyereisen, Silvia Spinelli, Stephen Stockdale, Mona Alqarni, Jennifer Mahony

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Christian Cambillau
University College Cork
Total Publications: 18
 
3
Marine Feyereisen
University College Cork
Total Publications: 6
 
4
Silvia Spinelli
University College Cork
Total Publications: 6
 
5
Stephen R. Stockdale
University College Cork
Total Publications: 15
 
6
Jennifer Mahony
University College Cork
Total Publications: 87