Type

Journal Article

Authors

Douwe van Sinderen
Arjen Nauta
Aldert Zomer
Horst Neve
Philip Kelleher
Jennifer Mahony
Francesca Bottacini
James Murphy

Subjects

Biochemistry

Topics
gene expression receptor binding protein comparative genomics lytic bacteriophage phage biology infection mechanism functional analysis sequence annotation

Comparative genomics and functional analysis of the 936 group of lactococcal Siphoviridae phages (2016)

Abstract Genome sequencing and comparative analysis of bacteriophage collections has greatly enhanced our understanding regarding their prevalence, phage-host interactions as well as the overall biodiversity of their genomes. This knowledge is very relevant to phages infecting Lactococcus lactis, since they constitute a significant risk factor for dairy fermentations. Of the eighty four lactococcal phage genomes currently available, fifty five belong to the so-called 936 group, the most prevalent of the ten currently recognized lactococcal phage groups. Here, we report the genetic characteristics of a new collection of 936 group phages. By combining these genomes to those sequenced previously we determined the core and variable elements of the 936 genome. Genomic variation occurs across the 936 phage genome, such as genetic elements that (i) lead to a +1 translational frameshift resulting in the formation of additional structures on the phage tail, (ii) specify a double neck passage structure, and (iii) encode packaging module-associated methylases. Hierarchical clustering of the gene complement of the 936 group phages and nucleotide alignments allowed grouping of the ninety 936 group phages into distinct clusters, which in general appear to correspond with their geographical origin.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
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Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Douwe van Sinderen, Arjen Nauta, Aldert Zomer, Horst Neve, Philip Kelleher, Jennifer Mahony, Francesca Bottacini, James Murphy

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
Arjen Nauta
University College Cork
Total Publications: 11
 
3
Aldert Zomer
University College Cork
Total Publications: 12
 
4
Horst Neve
University College Cork
Total Publications: 41
 
5
Philip Kelleher
University College Cork
 
6
Jennifer Mahony
University College Cork
Total Publications: 87
 
7
Francesca Bottacini
University College Cork
Total Publications: 32
 
8
James Murphy
University College Cork