Type

Journal Article

Authors

Daniel Kelly
Patrick Prendergast
Derek Morris
Paula Murphy
Elaine Kenny

Subjects

Biochemistry

Topics
wnt signalling zoology signalling pathways next generation medical devices cytoskeleton skeletal development differentially expressed genes spd transcriptome mechanical stimulation mechanosensitive genes genes society

Identification of mechanosensitive genes during skeletal development: Alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways (2014)

Abstract Background Mechanical stimulation is necessary for regulating correct formation of the skeleton. Here we test the hypothesis that mechanical stimulation of the embryonic skeletal system impacts expression levels of genes implicated in developmentally important signalling pathways in a genome wide approach. We use a mutant mouse model with altered mechanical stimulation due to the absence of limb skeletal muscle (Splotch-delayed) where muscle-less embryos show specific defects in skeletal elements including delayed ossification, changes in the size and shape of cartilage rudiments and joint fusion. We used Microarray and RNA sequencing analysis tools to identify differentially expressed genes between muscle-less and control embryonic (TS23) humerus tissue. Results We found that 680 independent genes were down-regulated and 452 genes up-regulated in humeri from muscle-less Spd embryos compared to littermate controls (at least 2-fold; corrected p-value ≤0.05). We analysed the resulting differentially expressed gene sets using Gene Ontology annotations to identify significant enrichment of genes associated with particular biological processes, showing that removal of mechanical stimuli from muscle contractions affected genes associated with development and differentiation, cytoskeletal architecture and cell signalling. Among cell signalling pathways, the most strongly disturbed was Wnt signalling, with 34 genes including 19 pathway target genes affected. Spatial gene expression analysis showed that both a Wnt ligand encoding gene (Wnt4) and a pathway antagonist (Sfrp2) are up-regulated specifically in the developing joint line, while the expression of a Wnt target gene, Cd44, is no longer detectable in muscle-less embryos. The identification of 84 genes associated with the cytoskeleton that are down-regulated in the absence of muscle indicates a number of candidate genes that are both mechanoresponsive and potentially involved in mechanotransduction, converting a mechanical stimulus into a transcriptional response. Conclusions This work identifies key developmental regulatory genes impacted by altered mechanical stimulation, sheds light on the molecular mechanisms that interpret mechanical stimulation during skeletal development and provides valuable resources for further investigation of the mechanistic basis of mechanoregulation. In particular it highlights the Wnt signalling pathway as a potential point of integration of mechanical and molecular signalling and cytoskeletal components as mediators of the response.
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Full list of authors on original publication

Daniel Kelly, Patrick Prendergast, Derek Morris, Paula Murphy, Elaine Kenny

Experts in our system

1
Daniel Kelly
Trinity College Dublin
Total Publications: 168
 
2
Patrick John Prendergast
Trinity College Dublin
Total Publications: 59
 
3
Derek Morris
Trinity College Dublin
Total Publications: 150
 
4
Paula Murphy
Trinity College Dublin
Total Publications: 42
 
5
Elaine Kenny
Trinity College Dublin
Total Publications: 28