Type

Journal Article

Authors

R. Paul Ross
Barry J. Plant
Fergus Shanahan
Colin Hill
Catherine Stanton
Orla O'Sullivan
Paul D. Cotter
Mary C Rea
M. J. Harrison
Daniel G. Burke
and 2 others

Subjects

Microbiology

Topics
cystic fibrosis transmembrane conductance regulator gut microbiota microbial diversity cystic fibrosis intestinal microbiota science and technology antibiotic therapy 454 pyrosequencing science foundation ireland sfi gastrointestinal tract

The altered gut microbiota in adults with cystic fibrosis (2017)

Abstract Background: Cystic Fibrosis (CF) is an autosomal recessive disease that affects the function of a number of organs, principally the lungs, but also the gastrointestinal tract. The manifestations of cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction in the gastrointestinal tract, as well as frequent antibiotic exposure, undoubtedly disrupts the gut microbiota. To analyse the effects of CF and its management on the microbiome, we compared the gut microbiota of 43 individuals with CF during a period of stability, to that of 69 non-CF controls using 454-pyrosequencing of the 16S rRNA gene. The impact of clinical parameters, including antibiotic therapy, on the results was also assessed. Results: The CF-associated microbiome had reduced microbial diversity, an increase in Firmicutes and a reduction in Bacteroidetes compared to the non-CF controls. While the greatest number of differences in taxonomic abundances of the intestinal microbiota was observed between individuals with CF and the healthy controls, gut microbiota differences were also reported between people with CF when grouped by clinical parameters including % predicted FEV1 (measure of lung dysfunction) and the number of intravenous (IV) antibiotic courses in the previous 12 months. Notably, CF individuals presenting with severe lung dysfunction (% predicted FEV1 ≤ 40%) had significantly (p < 0.05) reduced gut microbiota diversity relative to those presenting with mild or moderate dysfunction. A significant negative correlation (−0.383, Simpson’s Diversity Index) was also observed between the number of IV antibiotic courses and gut microbiota diversity. Conclusions: This is one of the largest single-centre studies on gut microbiota in stable adults with CF and demonstrates the significantly altered gut microbiota, including reduced microbial diversity seen in CF patients compared to healthy controls. The data show the impact that CF and it's management have on gut microbiota, presenting the opportunity to develop CF specific probiotics to minimise microbiota alterations.
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Full list of authors on original publication

R. Paul Ross, Barry J. Plant, Fergus Shanahan, Colin Hill, Catherine Stanton, Orla O'Sullivan, Paul D. Cotter, Mary C Rea, M. J. Harrison, Daniel G. Burke and 2 others

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
B J Plant
University College Cork
Total Publications: 35
 
3
Fergus Shanahan
University College Cork
Total Publications: 237
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Catherine Stanton
Teagasc
Total Publications: 261
 
6
Orla O'Sullivan
Teagasc
Total Publications: 92
 
7
Paul D. Cotter
Teagasc
Total Publications: 253
 
8
Mary C. Rea
Teagasc
Total Publications: 68
 
9
M J Harrison
Teagasc
Total Publications: 5
 
10
D G Burke
Teagasc
Total Publications: 5