Type

Journal Article

Authors

Conor Timothy Buckley
Veronica Ann Campbell
Daniel Kelly
Fergal J O'Brien
Tatiana Vinardell
S D Thorpe

Subjects

Engineering

Topics
cell culture techniques collagen type ii drug effects mesenchymal stromal cells cytology dynamic loading glycosaminoglycans compressive strength tissue engineering transforming growth factor beta3 swine anatomy mesenchymal stem cells pharmacology physiology chondrogenesis bioreactors dynamic strain dynamic compression tgf 3 animals metabolism

Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. (2008)

Abstract The objective of this study was to investigate the influence of dynamic compressive loading on chondrogenesis of mesenchymal stem cells (MSCs) in the presence of TGF-?3. Isolated porcine MSCs were suspended in 2% agarose and subjected to intermittent dynamic compression (10% strain) for a period of 42 days in a dynamic compression bioreactor. After 42 days in culture, the free-swelling specimens exhibited more intense alcian blue staining for proteoglycans, while immunohistochemical analysis revealed increased collagen type II immunoreactivity. Glycosaminoglycan (GAG) content increased with time for both free-swelling and dynamically loaded constructs, and by day 42 it was significantly higher in both the core (2.5 ? 0.21%w/w vs. 0.94 ? 0.03%w/w) and annulus (1.09 ? 0.09%w/w vs. 0.59 ? 0.08%w/w) of free-swelling constructs compared to dynamically loaded constructs. This result suggests that further optimization is required in controlling the biomechanical and/or the biochemical environment if such stimuli are to have beneficial effects in generating functional cartilaginous tissue.
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Full list of authors on original publication

Conor Timothy Buckley, Veronica Ann Campbell, Daniel Kelly, Fergal J O'Brien, Tatiana Vinardell, S D Thorpe

Experts in our system

1
Conor T Buckley
Trinity College Dublin
Total Publications: 91
 
2
Veronica Campbell
Trinity College Dublin
Total Publications: 19
 
3
Daniel Kelly
Trinity College Dublin
Total Publications: 190
 
4
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
 
5
Tatiana Vinardell
Trinity College Dublin
Total Publications: 27
 
6
Stephen D Thorpe
Trinity College Dublin
Total Publications: 18