Type

Journal Article

Authors

Andre Brodkorb
Colin Hill
Song Miao
Mary C Rea
Paula M O'Connor
Beatriz Gómez-Sala
Ronan Gough

Subjects

Microbiology

Topics
infectious diseases gram positive bacteria purification whey permeate cell culture cell free antimicrobial activity antimicrobial peptide lactic acid salting out nisin producing culture nisin

A Simple Method for the Purification of Nisin (2017)

Abstract Nisin, an antimicrobial peptide showing activity against a broad range of Gram-positive bacteria, is widely used as a food preservative and has potential as a therapeutic for a range of infectious diseases. Here, we present a simple purification method, based on a salting-out approach, which can produce a powder containing ∼33% nisin, from a nisin-producing culture in a whey permeate-based medium. This process removes over 99% of the lactic acid, NaCl, lactose and non-nisin proteins from the cell-free culture supernatant. The approach can also enrich a commonly used commercial nisin preparation over 30-fold to a purity of ∼58%. These are higher purities than comparable published methods. The simplicity of this approach facilitates its use in research and also its scale-up.
Collections Ireland -> Teagasc -> Food Chemistry & Technology

Full list of authors on original publication

Andre Brodkorb, Colin Hill, Song Miao, Mary C Rea, Paula M O'Connor, Beatriz Gómez-Sala, Ronan Gough

Experts in our system

1
Andre Brodkorb
Teagasc
Total Publications: 38
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Song Miao
Teagasc
Total Publications: 5
 
4
Mary C. Rea
Teagasc
Total Publications: 68
 
5
Paula M. O'Connor
Teagasc
Total Publications: 85
 
6
Beatriz Gómez-Sala
University College Cork
Total Publications: 3