Type

Journal Article

Authors

D.A Kenny
Patrick Lonergan
J.R Dick
Shauna A Holden
A.M English
Seán Fair
C.J Byrne

Subjects

Biology

Topics
fatty acid semen lipid composition safflower polyunsaturated fatty acids fish oil fertility pufa

Dietary polyunsaturated fatty acid supplementation of young post-pubertal dairy bulls alters the fatty acid composition of seminal plasma and spermatozoa but has no effect on semen volume or sperm quality (2017)

Abstract The full text of this article will not be available on ULIR until the embargo expires on the 18/12/2017 The aim of this study was to examine the effects of dietary supplementation with rumen protected n-6 or n-3 polyunsaturated fatty acids (PUFA) on the quantity and quality of semen from young post-pubertal dairy bulls. Pubertal Holstein-Friesian (n= ­ 43) and Jersey (n= 7) bulls with a mean ± s.e.m. age and bodyweight of 420.1 ± 5.86 days and 382 ± 8.94 kg, respectively, were blocked on breed, weight, age and semen quality (based on the outcomes of two pre-trial ejaculates) and randomly assigned to one of three treatments: (i) a non-supplemented control (CTL, n = 15), (ii) rumen-protected safflower (SO, n= 15), (iii) rumen-protected n-3 PUFA-enriched fish oil (FO, n = 20). Bulls were fed their respective diets, ad libitum for 12 weeks; individual intakes were recorded using an electronic feeding system for the initial 6 weeks of the feeding period. Semen was collected via electro-ejaculation at weeks 2, 1, 0, 7, 10, 11 and 12 relative to the beginning of the trial period (week 0). On collection, semen volume, sperm concentration and progressive linear motility (PLM) were assessed. On weeks 2, 1, 0, 10, 11, 12, semen was packaged into 0.25 mL straws and frozen using a programmable freezer. On weeks 1, 7 and 11; a subsample of semen was separated into sperm and seminal plasma, by centrifugation and stored at e 20 C until analysis of lipid composition. Semen from 10 bulls per treatment were used for post-thaw analysis at weeks 10, 11 and 12 (3 straws per ejaculate). Sperm motility was analysed by computer assisted semen analysis (CASA). In addition, membrane fluidity, acrosome reaction and oxidative stress were assessed using flow cytometry. Sperm from bulls fed SO had a 1.2 fold higher total n-6 PUFA content at week 11 compared to week 1 (P < 0.01) while bulls fed FO had a 1.3 fold higher total n-3 PUFA content, in sperm by week 11 (P < 0.01). There was no effect of diet on semen volume, concentration or PLM of sperm when assessed either immediately following collection or post-thawing. Membrane fluidity and oxidative stress of spermwere also not affected by diet. The percentage of sperm with intact-acrosomes was lower in CTL bulls compared to those fed SO (P < 0.01). In conclusion, while the lipid composition of semen was altered following dietary supplementation with either n-6 or n-3 based PUFA, this did not lead to measurable improvements in the quantity or quality of semen produced by young post-pubertal dairy bulls.
Collections Ireland -> University of Limerick -> Biological Sciences
Ireland -> University of Limerick -> Faculty of Science and Engineering
Ireland -> University of Limerick -> Departments Science and Engineering

Full list of authors on original publication

D.A Kenny, Patrick Lonergan, J.R Dick, Shauna A Holden, A.M English, Seán Fair, C.J Byrne

Experts in our system

1
D.A. Kenny
Teagasc
Total Publications: 138
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
Shauna A Holden
University of Limerick
Total Publications: 13
 
4
A M English
Teagasc
Total Publications: 11
 
5
Seán Fair
University of Limerick
Total Publications: 54
 
6
C J Byrne
Teagasc
Total Publications: 11