Type

Journal Article

Authors

Seán Fair
P Longeran
S.T Butler
L Brennan
A O'Gorman
H Whelan
C Murphy
B Fermandez-Fuertes
Shauna A Holden

Subjects

Veterinary

Topics
fertility bovine glutamic acid gas chromatography mass spectrometry flow cytometry fatty acid metabolomics sperm

Relationship between in vitro sperm functional assessments, seminal plasma composition, and field fertility after AI with either non-sorted or sex-sorted bull semen (2017)

Abstract The full text of this article will not be available on ULIR until the embargo expires on the 2/1/2018 The hypothesis of this study was that different in vitro parameters are required to predict the in vivo fertility of non-sorted (NS) and sex-sorted (SS) semen. Thus, the aim was to correlate in vitro bull sperm functional parameters (experiment 1) and seminal plasma composition (experiment 2) with pregnancy rates using 2 cohorts of bulls (NS and SS). Experiment 1: ejaculates from each bull (n = 3 ejaculates per bull; n = 6 bulls for both NS and SS) were assessed for motility, thermal stress tolerance and morphology using microscopy, and viability, osmotic resistance, mitochondrial membrane potential, and acrosome integrity using flow cytometry. Fertilizing ability was assessed using IVF. Experiment 2: ejaculates (n = 3 per bull; n = 8 and 6 bulls for NS and SS, respectively) were collected, seminal plasma harvested and frozen and later analyzed for amino acid and fatty acid composition using gas chromatography mass spectrometry. In the NS cohort of bulls, there was no correlation between pregnancy rate and any of the sperm functional parameters assessed. However, within the SS cohort, motility and viability were correlated with pregnancy rate (r = 0.84 and 0.80, respectively; P < 0.05). There was no correlation between IVF outcome and pregnancy rate in either the SS or NS cohort of bulls. In the NS cohort of bulls, concentrations of the amino acid isoleucine and the fatty acid tricosylic acid (C23:0) were correlated with pregnancy rate (r = 0.80 and 0.74, respectively; P < 0.05). Within the SS cohort of bulls, the amino acid glutamic acid and the fatty acid arachidic acid (C20:0) were correlated with pregnancy rate (r = 0.84 and 0.82, respectively; P < 0.05). In conclusion, this study suggests that different in vitro markers of fertility are required to predict the fertility of NS and SS sperm.
Collections Ireland -> University of Limerick -> Biological Sciences
Ireland -> University of Limerick -> Faculty of Science and Engineering
Ireland -> University of Limerick -> Departments Science and Engineering

Full list of authors on original publication

Seán Fair, P Longeran, S.T Butler, L Brennan, A O'Gorman, H Whelan, C Murphy, B Fermandez-Fuertes, Shauna A Holden

Experts in our system

1
Seán Fair
University of Limerick
Total Publications: 68
 
2
S T Butler
Teagasc
Total Publications: 89
 
3
Lorraine Brennan
University College Dublin
Total Publications: 166
 
4
Aoife O'Gorman
University College Dublin
Total Publications: 13
 
5
C Murphy
University of Limerick
Total Publications: 14
 
6
Shauna A Holden
University of Limerick
Total Publications: 14