Type

Journal Article

Authors

Tatiana Vinardell
Daniel Kelly
Conor Buckley
S D Thorpe

Subjects

Biochemistry

Topics
animals mesenchymal stem cell transplantation cartilage articular injuries push out test cells cultured chondrogenesis next generation medical devices integration chondrocytes transplantation tgf 3 pathology physiology stem cells cytology swine in vitro techniques surgery neuroscience

Chondrogenesis and integration of mesenchymal stem cells within an in vitro cartilage defect repair model (2009)

Abstract Integration of repair tissue is a key indicator of the long-term success of cell-based therapies for cartilage repair. The objective of this study was to compare the in vitro chondrogenic differentiation and integration of agarose hydrogels seeded with either chondrocytes or bone marrow derived mesenchymal stem cells (MSCs) in defects created in cartilage explants. Chondrocytes and MSCs were isolated from porcine donors, suspended in 2% agarose and then injected into cylindrical defects within the explants. These constructs were maintained in a chemically defined medium supplemented with 10 ng/ml of TGF-?3. Cartilage integration was assessed by histology and mechanical push-out tests. After 6 weeks in culture, chondrocyte seeded constructs demonstrated a higher integration strength (64.4 ? 8.3 kPa) compared to MSC seeded constructs (22.7 ? 5.9 kPa). GAG (1.27 ? 0.3 kPa vs 0.19 ? 0.03 kPa) and collagen (0.31 ? 0.08 kPa vs 0.09 ? 0.01 kPa) accumulation in chondrocyte seeded constructs was greater than that measured in the MSC seeded group. The GAG, collagen and DNA content of both chondrocyte and MSC-seeded hydrogels cultured in cartilage explants was significantly lower than control constructs cultured in free swelling conditions. The results of this study suggest that the explant model may constitute a more rigorous in vitro test to assess MSC therapies for cartilage defect repair.
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Full list of authors on original publication

Tatiana Vinardell, Daniel Kelly, Conor Buckley, S D Thorpe

Experts in our system

1
Tatiana Vinardell
Trinity College Dublin
Total Publications: 27
 
2
Daniel Kelly
Trinity College Dublin
Total Publications: 168
 
3
Conor T Buckley
Trinity College Dublin
Total Publications: 85
 
4
Stephen D Thorpe
Trinity College Dublin
Total Publications: 18