Type

Journal Article

Authors

P Ronan O'Connell
Desmond C. Winter
J Calvin Coffey
Kieran Sheahan
Fergus Shanahan
John M Hyland
Diarmuid O Donoghue
Glen Doherty
Hugh E Mulcahy
Aine Balfe
and 5 others

Subjects

Microbiology

Topics
female enterotypes gut microbiota genetics disease colonoscopy male mucous membrane intestinal microbiota isolation purification segmentation instrumentation microbiota middle aged aged stability bacteria human colonic microbiota psychiatry colonic microbiota polymorphism restriction fragment length ecosystem diversity ulcerative colitis healthy volunteers dna bacterial microbiology colon biodiversity community health tract classification surgical instruments bacteriology amplified fragment length polymorphism analysis therapy human health flora rna ribosomal 16s genetic variation humans adult spatial ecology spatial micorbial assessment cluster analysis

Depth-Dependent Differences in Community Structure of the Human Colonic Microbiota in Health (2013)

Abstract Objective The aims of this study were to develop techniques for spatial microbial assessment in humans and to establish colonic luminal and mucosal spatial ecology, encompassing longitudinal and cross-sectional axes. Design A microbiological protected specimen brush was used in conjunction with a biopsy forceps to sample the colon in nine healthy volunteers undergoing colonoscopy. Terminal Restriction Fragment Length Polymorphism analysis was used to determine the major variables in the spatial organization of the colonic microbiota. Results Protected Specimen Brush sampling retrieved region-specific, uncontaminated samples that were enriched for bacterial DNA and depleted in human DNA when compared to biopsy samples. Terminal Restriction Fragment Length Polymorphism analysis revealed a segmentation of bacterial communities between the luminal brush and biopsy-associated ecological niches with little variability across the longitudinal axis of the colon and reduced diversity in brush samples. Conclusion These results support the concept of a microbiota with little longitudinal variability but with some degree of segregation between luminal and mucosal communities.
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Full list of authors on original publication

P Ronan O'Connell, Desmond C. Winter, J Calvin Coffey, Kieran Sheahan, Fergus Shanahan, John M Hyland, Diarmuid O Donoghue, Glen Doherty, Hugh E Mulcahy, Aine Balfe and 5 others

Experts in our system

1
P R O'Connell
University College Dublin
Total Publications: 75
 
2
Desmond C. Winter
University College Dublin
Total Publications: 53
 
3
J Calvin Coffey
University of Limerick
Total Publications: 72
 
4
Kieran Sheahan
University College Dublin
Total Publications: 40
 
5
Fergus Shanahan
University College Cork
Total Publications: 259
 
6
John Hyland
Dublin Business School
Total Publications: 47
 
7
Glen A Doherty
University College Dublin
Total Publications: 57
 
8
Hugh E Mulcahy
University College Dublin
Total Publications: 43
 
9
Aine Balfe
University of Limerick