Type

Journal Article

Authors

Kevin Kavanagh
Martin Clynes
James C. Carolan
Clair Gallagher
Amie Maher
Carla Surlis
Niall Browne

Subjects

Microbiology

Topics
increases infection larvae candida albicans bacterial staphylococcus aureus susceptibility malate dehydrogenase

Prolonged pre-incubation increases the susceptibility of Galleria mellonella larvae to bacterial and fungal infection (2015)

Abstract Galleria mellonella larvae are widely used for assessing the virulence of microbial pathogens and for measuring the in vivo activity of antimicrobial agents and produce results comparable to those that can be obtained using mammals. The aim of the work described here was to ascertain the effect of pre-incubation at 15�C for 1, 3, 6 or 10 weeks on the susceptibility of larvae to infection with Candida albicans and Staphylococcus aureus. Larvae infected with C. albicans after 1 week pre-incubation at 15�C showed 73.3 § 3.3% survival at 24 hours post-infection while those infected after 10 weeks pre-incubation showed 30 § 3.3% survival (P < 0.01). Larvae infected with S. aureus after 1 week preincubation showed 65.5 § 3.3% survival after 24 hours while those infected after 10 weeks pre-incubation showed 13.3 § 3.3% (P < 0.001). Analysis of the haemocyte density in larvae pre-incubated for 3–10 weeks showed a reduction in haemocytes over time but a proportionate increase in the density of granular haemocytes in the population as determined by FACS analysis. Proteomic analysis revealed decreased abundance of proteins associated with metabolic pathways (e.g. malate dehydrogenase, fructose-1,6-bisphosphatase, glyceraldehyde-3-phosphate dehydrogenase) and prophenoloxidase. G. mellonella larvae are a useful in vivo model system but the duration of the pre-incubation stage significantly affects their susceptibility to microbial pathogens possibly as a result of altered metabolism.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes: Institute of Immunology
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Kevin Kavanagh, Martin Clynes, James C. Carolan, Clair Gallagher, Amie Maher, Carla Surlis, Niall Browne

Experts in our system

1
Kevin Kavanagh
Maynooth University
Total Publications: 139
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
James C. Carolan
Maynooth University
Total Publications: 28
 
4
Clair Gallagher
Dublin City University
Total Publications: 12
 
5
Carla Surlis
Maynooth University
Total Publications: 6
 
6
Niall Browne
Maynooth University
Total Publications: 7