Type

Conference Proceedings

Authors

Fiona Regan
Jens Ducree
Richard O'Kennedy
Charles Nwankire
Caroline Murphy
Brendan Heery
Jenny Fitzgerald
Ivan Maguire

Subjects

Chemistry

Topics
load immunofluorescence detection recombinant antibody technology low cost diagnostic device toxin detection lab on a disc analytical chemistry chemistry microcystin microfluidics

The ToxiSense detection system: A novel centrifugal-based microfluidic (Lab-On-A-Disc) system for detecting cyanobacterial toxin microcystin-LR (2015)

Abstract Cyclic peptide cyanobacterial toxins, in particular Microcystis aeruginosa, pose a serious health risk to humans and animals alike [1], [2]. Occurring mostly in fresh and brackish water, they have been identified to cause cancer promotion and liver damage [3]. Herein, we describe a portable, microfluidic-based system for in-situ detection of algal toxins in fresh water. The Atalanta system is a novel, portable and sample-to-answer platform for the detection of toxic cyanobacteria – Microcystin-LR in fresh water. Atalanta utilises the partnership of highly-specific recombinant chicken anti-microcystin antibodies, prepared in-house, with a 3D-printed ‘LASER-photo¬diode’ fluorescent detection method, also developed in-house. A competitive immunoassay format is utilised to detect free toxin. Furthermore, dissolvable-film (DF) based flow-actuation facilitates full assay inte¬gration. This new approach will form the basis of a cost efficient, USB-controlled water quality monitoring system. The Atalanta detection system consists of two components; the microfluidic Atalanta disc and the disc-holder. The Atalanta disc-holder was fabricated and assembled from a 3D-printed casing, with electronic components housed in device. The 5-layered microfluidic disc consists of five reservoirs, each with a separate venti¬lation, aligned radially with inter-connected microchannels. Each reservoir represents a functional assay step. First, microcystin conjugate is coated to the functionalised surface of the reservoir 3 prior to assembling the disc. A freshwater sample in reservoir 1 is pre-incubated with recombinant antibodies labelled with fluorophore (Alexa 647) in reservoir 2. This is then spun into reservoir 3 for detection through a competitive immunoassay using Microcystin-LR. Low fluorescence signal indicates high Microcystin-LR concentration in the sample.
Collections Ireland -> Dublin City University -> Publication Type = Conference or Workshop Item
Ireland -> Dublin City University -> Subject = Physical Sciences: Analytical chemistry
Ireland -> Dublin City University -> Subject = Physical Sciences: Chemistry
Ireland -> Dublin City University -> Status = Unpublished
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Chemical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: Marine and Environmental Sensing Technology Hub (MESTECH)
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Physical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology

Full list of authors on original publication

Fiona Regan, Jens Ducree, Richard O'Kennedy, Charles Nwankire, Caroline Murphy, Brendan Heery, Jenny Fitzgerald, Ivan Maguire

Experts in our system

1
Fiona Regan
Dublin City University
Total Publications: 101
 
2
Jens Ducree
Dublin City University
Total Publications: 83
 
3
R O'Kennedy
Dublin City University
Total Publications: 197
 
4
Charles Nwankire
Dublin City University
Total Publications: 22
 
5
Caroline Murphy
Dublin City University
Total Publications: 12
 
6
Brendan Heery
Dublin City University
Total Publications: 31
 
7
Jenny Fitzgerald
Dublin City University
Total Publications: 18
 
8
Ivan Maguire
Dublin City University
Total Publications: 15