Type

Journal Article

Authors

Fergal J O'Brien
Thomas C Flanagan
Stefan Jockenhoevel
Gerard M Cooney
Nian Shen
Tanya J Levingstone
Claire M Brougham

Subjects

Engineering

Topics
parameters natural scaffolds microarchitecture collagen tissue engineering freeze drying controlled

Freeze-Drying as a Novel Biofabrication Method for Achieving a Controlled Microarchitecture within Large, Complex Natural Biomaterial Scaffolds. (2017)

Abstract The biofabrication of large natural biomaterial scaffolds into complex 3D shapes which have a controlled microarchitecture remains a major challenge. Freeze-drying (or lyophilization) is a technique used to generate scaffolds in planar 3D geometries. Here we report the development of a new biofabrication process to form a collagen-based scaffold into a large, complex geometry which has a large height to width ratio, and a controlled porous microarchitecture. This biofabrication process is validated through the successful development of a heart valve shaped scaffold, fabricated from a collagen-glycosaminoglycan co-polymer. Notably, despite the significant challenges in using freeze-drying to create such a structure, the resultant scaffold has a uniform, homogenous pore architecture throughout. This is achieved through optimization of the freeze-drying mold and the freezing parameters. We believe this to be the first demonstration of using freeze-drying to create a large, complex scaffold geometry with a controlled, porous architecture for natural biomaterials. This study validates the potential of using freeze-drying for development of organ-specific scaffold geometries for tissue engineering applications, which up until now might not have been considered feasible.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Fergal J O'Brien, Thomas C Flanagan, Stefan Jockenhoevel, Gerard M Cooney, Nian Shen, Tanya J Levingstone, Claire M Brougham

Experts in our system

1
Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 265
 
2
Thomas C Flanagan
Dublin Institute of Technology
Total Publications: 5
 
3
Stefan Jockenhoevel
Dublin Institute of Technology
Total Publications: 6
 
4
Gerard Cooney
Dublin Institute of Technology
Total Publications: 3
 
5
Nian Shen
Royal College of Surgeons in Ireland
Total Publications: 3
 
6
Tanya J Levingstone
Royal College of Surgeons in Ireland
Total Publications: 28
 
7
Claire M Brougham
Dublin Institute of Technology
Total Publications: 9