Type

Journal Article

Authors

Paula Meleady
Martin Clynes
Orla Coleman
Michael Henry

Subjects

Biochemistry

Topics
proteins site specific cho cells chinese hamster ovary cells apoptosis post translational modifications recombinant signal transduction

Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells. (2017)

Abstract The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues is one of the most important post-translational modifications that regulates many biological processes. The phosphoproteome has not been studied in any great detail in recombinant Chinese hamster ovary (CHO) cells to date despite phosphorylation playing a crucial role in regulating many molecular and cellular processes relevant to bioprocess phenotypes including, for example, transcription, translation, growth, apoptosis, and signal transduction. In this chapter, we provide a protocol for the phosphoproteomic analysis of Chinese hamster ovary cells using phosphopeptide enrichment with metal oxide affinity chromatography (MOAC) and immobilized metal affinity chromatography (IMAC) techniques, followed by site-specific identification of phosphorylated residues using LC-MS (MS2 and MS3) strategies.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Paula Meleady, Martin Clynes, Orla Coleman, Michael Henry

Experts in our system

1
Paula Meleady
Dublin City University
Total Publications: 95
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Orla Coleman
Dublin City University
Total Publications: 6
 
4
Michael Henry
Dublin City University
Total Publications: 78