Type

Journal Article

Authors

Paula Meleady
Martin Clynes
Michael Henry
Orla Coleman

Subjects

Biochemistry

Topics
biopharmaceutical production recombinant proteins cho cells chinese hamster ovary cells cell line proteome filter sample preparation

Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells. (2017)

Abstract Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell line for biopharmaceutical production because of their ability to correctly fold and posttranslationally modify recombinant proteins that are compatible with human use. Proteomics, along with other 'omic platforms, are being used to understand the biology of CHO cells with the ultimate aim of enhancing CHO cell factories for more efficient production of biopharmaceuticals. In this chapter, we will describe an efficient protocol called Filter Aided Sample Preparation (FASP) for the extraction of proteins from CHO cells for proteomic studies. FASP uses a common ultrafiltration device whereby the membrane pores are small enough to allow contaminating detergents to pass through, while proteins are too large and are retained and concentrated in the filter unit. This method of sample preparation and protein digestion is universally applicable and can be easily employed in any proteomics facilities as standard everyday laboratory reagents and equipment are used.
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Full list of authors on original publication

Paula Meleady, Martin Clynes, Michael Henry, Orla Coleman

Experts in our system

1
Paula Meleady
Dublin City University
Total Publications: 95
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Orla Coleman
Dublin City University
Total Publications: 6