Type

Journal Article

Authors

Niall Barron
Martin Clynes
Nga Lao
Alan Costello

Subjects

Biochemistry

Topics
binding sites vectors cho cells oligonucleotides antisense chinese hamster ovary cells generation cell lines micrornas

Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors. (2017)

Abstract MicroRNAs (miRNAs) are small, noncoding RNAs of about 22 nucleotides in length and have proven to be useful targets for genetic modifications for desirable phenotype in the biotech industry. The use of constitutively expressed "miRNA sponge" vectors in which multiple, tandem miRNA binding sites containing transcripts are transcriptionally regulated by a constitutive promoter for down regulating the levels of endogenous microRNAs in Chinese hamster ovary (CHO) cells has shown to be more advantageous than using synthetic antisense oligonucleotides. The application of miRNA sponges in biotechnological processes, however, could be more effective, if expression of miRNA sponges could be tuned. In this chapter, we present a method for the generation of stable CHO cell lines expressing a TET-ON-SanDI-miRNA-sponge that is in theory expressed only in the presence of an inducer.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Niall Barron, Martin Clynes, Nga Lao, Alan Costello

Experts in our system

1
Niall Barron
Dublin City University
Total Publications: 44
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Nga T Lao
Dublin City University
Total Publications: 7
 
4
Alan Costello
Dublin City University
Total Publications: 3