Type

Journal Article

Authors

Fionnuala Ní Áinle
Johan W M Heemskerk
Richard O'Kennedy
Kieran Wynne
Patricia Maguire
Martin Parsons
Elaine Neary
Seamus Allen
Aine Lennon
Barry Kevane
and 3 others

Subjects

Biochemistry

Topics
factor viii surface plasmon resonance factor xa plasmon resonance spr factor ix platelet factor viia protein c

Effect of platelet-derived β-thromboglobulins on coagulation. (2016)

Abstract β-thromboglobulins are derived from the cleavage of the CXC chemokine platelet basic protein and are released in high concentrations by activated platelets. Platelet-derived β-thromboglobulins (βTG) share 70% homology with platelet factor 4 (PF4), another CXC chemokine released by activated platelets. PF4 modulates coagulation by inhibiting heparin-antithrombin interactions, promoting protein C activation, and attenuating the activity of activated protein C. In contrast, the effect of βTG on coagulation is unknown. Clotting times, thrombin generation, chromogenic clotting factor assays, and surface plasmon resonance (SPR) were used to assess the effect of purified βTG on coagulation. In normal pooled plasma, βTG shortened the lagtime and time to peak thrombin generation of tissue factor (TF)-dependent and TF-independent thrombin generation. In factor VIII and factor IX-deficient plasmas, βTG induced thrombin generation in the absence of a TF stimulus and in the presence of anti-TF and factor VIIa inhibitory antibodies. The procoagulant effect was not observed when thrombin generation was independent of factor X activation (supplementation of factor X-deficient plasma with factor Xa). Cleavage of a factor Xa-specific chromogenic substrate was observed when βTG was incubated with factor X, suggesting a direct interaction between βTG and factor X. Using SPR, βTG were found to bind to immobilised factor X in a dose dependent manner. βTG modulate coagulation in vitro via an interaction with factor X.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Fionnuala Ní Áinle, Johan W M Heemskerk, Richard O'Kennedy, Kieran Wynne, Patricia Maguire, Martin Parsons, Elaine Neary, Seamus Allen, Aine Lennon, Barry Kevane and 3 others

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Kieran Wynne
Royal College of Surgeons in Ireland
Total Publications: 43
 
3
P B Maguire
University College Dublin
Total Publications: 31
 
4
Martin Parsons
University College Dublin
Total Publications: 3