Journal Article


Tia E Keyes
Robert J Forster
Niamh Moran
Una Prendergast
Elaine Spain
Kellie Adamson



blood platelets surface enhanced raman spectroscopy chemical modification surface geometry self assembled monolayers sams aspartic acid gold arrays

Peptide-Mediated Platelet Capture at Gold Micropore Arrays. (2016)

Abstract Ordered spherical cap gold cavity arrays with 5.4, 1.6, and 0.98 μm diameter apertures were explored as capture surfaces for human blood platelets to investigate the impact of surface geometry and chemical modification on platelet capture efficiency and their potential as platforms for surface enhanced Raman spectroscopy of single platelets. The substrates were chemically modified with single-constituent self-assembled monolayers (SAM) or mixed SAMs comprised of thiol-functionalized arginine-glycine-aspartic acid (RGD, a platelet integrin target) with or without 1-octanethiol (adhesion inhibitor). As expected, platelet adhesion was promoted and inhibited at RGD and alkanethiol modified surfaces, respectively. Platelet adhesion was reversible, and binding efficiency at the peptide modified substrates correlated inversely with pore diameter. Captured platelets underwent morphological change on capture, the extent of which depended on the topology of the underlying substrate. Regioselective capture of the platelets enabled study for the first time of the surface enhanced Raman spectroscopy of single blood platelets, yielding high quality Raman spectroscopy of individual platelets at 1.6 μm diameter pore arrays. Given the medical importance of blood platelets across a range of diseases from cancer to psychiatric illness, such approaches to platelet capture may provide a useful route to Raman spectroscopy for platelet related diagnostics.
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Full list of authors on original publication

Tia E Keyes, Robert J Forster, Niamh Moran, Una Prendergast, Elaine Spain, Kellie Adamson

Experts in our system

Tia E. Keyes
Dublin City University
Total Publications: 104
Robert J. Forster
Dublin City University
Total Publications: 92
Niamh Moran
Royal College of Surgeons in Ireland
Total Publications: 50
Úna Prendergast
Dublin City University
Total Publications: 4
Elaine Spain
Dublin City University
Total Publications: 15
Kellie Adamson
Dublin City University
Total Publications: 8