Type

Journal Article

Authors

Annemarie Larkin
Patrick Ormond
Bairbre Wynne
Mary Monks
Niamh Leonard
Mairin McMenamin
Martin Clynes
Michael Henry
Paula Meleady
Edel McAuley
and 2 others

Subjects

Pharmacology

Topics
fatty acid quantitative analysis mass spectrometry kinase 3 tissue analysis malignant melanoma tandem mass spectrometry medical records

Quantitative label-free mass spectrometry analysis of formalin-fixed, paraffin-embedded tissue representing the invasive cutaneous malignant melanoma proteome. (2016)

Abstract Understanding the events at a protein level that govern the progression from melanoma in situ to invasive melanoma are important areas of current research to be developed. Recent advances in the analysis of formalin-fixed, paraffin-embedded tissue by proteomics, particularly using the filter-aided sample preparation protocol, has opened up the possibility of studying vast archives of clinical material and associated medical records. In the present study, quantitative protein profiling was performed using tandem mass spectrometry, and the proteome differences between melanoma in situ and invasive melanoma were compared. Biological pathway analyses revealed several signalling pathways differing between melanoma in situ and invasive melanoma, including metabolic pathways and the phosphoinositide 3-kinase-Akt signalling pathway. Selected proteins of interest (14-3-3ε and fatty acid synthase) were subsequently investigated using immunohistochemical analysis of tissue microarrays. Identifying the key proteins that play significant roles in the establishment of a more invasive phenotype in melanoma may ultimately aid diagnosis and treatment decisions.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Annemarie Larkin, Patrick Ormond, Bairbre Wynne, Mary Monks, Niamh Leonard, Mairin McMenamin, Martin Clynes, Michael Henry, Paula Meleady, Edel McAuley and 2 others

Experts in our system

1
Annemarie Larkin
Dublin City University
Total Publications: 25
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Paula Meleady
Dublin City University
Total Publications: 95