Type

Journal Article

Authors

R O'Kennedy
H Ma

Subjects

Microbiology

Topics
recombinant antibodies recombinant antibody production construction generation escherichia coli single metal expression

Recombinant antibody fragment production. (2016)

Abstract Recombinant antibodies are now very important in both therapeutics and diagnostics and offer significant advantages over conventional antibodies. The generation of a single-chain variable antibody fragment (scFv) (a common and important recombinant antibody format) is used to demonstrate the construction of a recombinant antibody library. An immunotube-based two-day panning approach, using Escherichia coli as an expression system, is utilised for antibody screening. The methods used for antibody selection and purification using immobilised metal affinity chromatography (IMAC) are described.
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Full list of authors on original publication

R O'Kennedy, H Ma

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Hui Ma
Dublin City University
Total Publications: 8