Type

Journal Article

Authors

Simon Wong
Dermot Kenny
Antonio J Ricco
Nigel Kent
Barry Byrne
Eimear Dunne
Hannah Dunne
Emma Hogan
Bruno Voisin
Jonathan Cowman
and 2 others

Subjects

Mathematics

Topics
blood tracking von willebrand factor software computational algorithms shear platelet

Computational Tracking of Shear-Mediated Platelet Interactions with von Willebrand Factor. (2016)

Abstract The imaging of shear-mediated dynamic platelet behavior interacting with surface-immobilized von Willebrand factor (vWF) has tremendous potential in characterizing changes in platelet function for clinical diagnostics purposes. However, the imaging output, a series of images representing platelets adhering and rolling on the surface, poses unique, non-trivial challenges for software algorithms that reconstruct the positional trajectories of platelets. We report on an algorithm that tracks platelets using the output of such flow run experiments, taking into account common artifacts encountered by previously-published methods, and we derive seven key metrics of platelet dynamics that can be used to characterize platelet function. Extensive testing of our method using simulated platelet flow run data was carried out to validate our tracking method and derived metrics in capturing key platelet-vWF interaction-dynamics properties. Our results show that while the number of platelets present on the imaged area is the leading cause of errors, flow run data from two experiments using whole blood samples showed that our method and metrics can detect platelet property changes/differences that are concordant with the expected biological outcome, such as inhibiting key platelet receptors such as P2Y1, glycoprotein (GP)Ib and GPIIb/IIIa. These findings support the use of our methodologies to characterize platelet function among a wide range of healthy and disease cohorts.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Simon Wong, Dermot Kenny, Antonio J Ricco, Nigel Kent, Barry Byrne, Eimear Dunne, Hannah Dunne, Emma Hogan, Bruno Voisin, Jonathan Cowman and 2 others

Experts in our system

1
Dermot Kenny
Royal College of Surgeons in Ireland
 
2
Antonio J. Ricco
Dublin City University
Total Publications: 24
 
3
Nigel J Kent
Dublin City University
Total Publications: 18
 
4
Barry Byrne
Dublin City University
 
5
Eimear Dunne
Royal College of Surgeons in Ireland
Total Publications: 19
 
6
Jonathan Cowman
Dublin City University
Total Publications: 3