Type

Journal Article

Authors

Paula Meleady
Michael Henry

Subjects

Biochemistry

Topics
liquid chromatography mass spectrometry mass spectrometry lc ms chromatography reverse phase liquid chromatography lc purification protein characterization two dimensional clinical proteomics

Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC-MS) Purification Systems. (2016)

Abstract Liquid chromatography-mass spectrometry (LC-MS) has become a routine powerful technology in clinical proteomic studies for protein identification, protein characterization and the discovery of biomarkers. In this chapter, we describe two protocol methods to analyze clinical patient samples using a resin based depletion column followed by either protein In-gel enzymatic digestion or protein in-solution enzymatic digestion and then analysis by one-dimensional reverse-phase chromatography or two-dimensional strong cation exchange (SCX)-reverse-phase chromatography (RPC).
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Paula Meleady, Michael Henry

Experts in our system

1
Paula Meleady
Dublin City University
Total Publications: 115
 
2
Michael Henry
Dublin City University
Total Publications: 99