Journal Article


Catherine M Greene
Eleanor J Molloy
Victoria McEneaney
Paul J McKiernan
Chiara De Santi
David N O'Driscoll



protein expression tyrosine kinase il 1 toll like receptor 4 x chromosome genes x linked gene expression signaling toll like receptor

Expression of X-linked Toll-like receptor 4 signaling genes in female vs. male neonates. (2016)

Abstract Male neonates display poorer disease prognosis and outcomes compared with females. Immune genes which exhibit higher expression in umbilical cord blood (UCB) of females may contribute to the female immune advantage during infection and inflammation. The aim of this study was to quantify expression of Toll-like receptor (TLR) 4 signaling genes encoded on the X-chromosome in UCB from term female vs. male neonates. UCB samples were collected from term neonates (n = 26) born by elective Caesarean section and whole blood was collected from adults (n = 20). Leukocyte RNA was isolated and used in quantitative PCR reactions for IκB kinase γ (IKKγ), Bruton's tyrosine kinase (BTK), and IL-1 receptor associated kinase (IRAK)1. IRAK1 protein was analyzed by Western blot and confocal microscopy. In neonates there was no significant difference in the relative expression of IKKγ or BTK mRNA between genders. IRAK1 gene and protein expression was significantly higher in female vs. male UCB, with increased cytosolic IRAK1 expression also evident in female UCB mononuclear cells. Adults had higher expression of all three genes compared with neonates. Increased expression of IRAK1 could be responsible, in part, for sex-specific responses to infection and subsequent immune advantage in female neonates.
Collections Ireland -> TU Dublin (Tallaght Campus) -> PubMed

Full list of authors on original publication

Catherine M Greene, Eleanor J Molloy, Victoria McEneaney, Paul J McKiernan, Chiara De Santi, David N O'Driscoll

Experts in our system

Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
Eleanor J Molloy
TU Dublin (Tallaght Campus)
Total Publications: 22
Paul J McKiernan
Royal College of Surgeons in Ireland
Total Publications: 13
David N O'Driscoll
Maynooth University
Total Publications: 3