Type

Journal Article

Authors

Jochen H M Prehn
Beau Fenner
Orla Watters
Sinéad Kinsella
Silke Andresen
Robert Schwamborn
Hans-Georg König

Subjects

Biochemistry

Topics
neurons gene silencing transcription factor chromatin immunoprecipitation nf b protein expression negative feedback

NF-κB regulates neuronal ankyrin-G via a negative feedback loop. (2015)

Abstract The axon initial segment (AIS) is a neuronal compartment defined by ankyrin-G expression. We here demonstrate that the IKK-complex co-localizes and interacts with the cytoskeletal anchor protein ankyrin-G in immunoprecipitation and proximity-ligation experiments in cortical neurons. Overexpression of the 270 kDa variant of ankyrin-G suppressed, while gene-silencing of ankyrin-G expression increased nuclear factor-κB (NF-κB) activity in primary neurons, suggesting that ankyrin-G sequesters the transcription factor in the AIS. We also found that p65 bound to the ank3 (ankyrin-G) promoter sequence in chromatin immunoprecipitation analyses thereby increasing ank3 expression and ankyrin-G levels at the AIS. Gene-silencing of p65 or ankyrin-G overexpression suppressed ank3 reporter activity. Collectively these data demonstrate that p65/NF-κB controls ankyrin-G levels via a negative feedback loop, thereby linking NF-κB signaling with neuronal polarity and axonal plasticity.
Collections Ireland -> Royal College of Surgeons in Ireland -> PubMed

Full list of authors on original publication

Jochen H M Prehn, Beau Fenner, Orla Watters, Sinéad Kinsella, Silke Andresen, Robert Schwamborn, Hans-Georg König

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Beau J Fenner
Royal College of Surgeons in Ireland
Total Publications: 9
 
3
Orla Watters
Royal College of Surgeons in Ireland
Total Publications: 5
 
4
Sinéad Kinsella
Royal College of Surgeons in Ireland
Total Publications: 10
 
5
Robert F Schwamborn
Royal College of Surgeons in Ireland
Total Publications: 6
 
6
Hans Georg König
Royal College of Surgeons in Ireland
Total Publications: 22