Type

Journal Article

Authors

Miguel Gueimonde
Marco Ventura
Clara G de Los Reyes-Gavilán
Ana M Hernández-Barranco
Lidia Alaez
Nuria Fernández
Gonzalo Solís
Silvia Arboleya
Christian Milani
Marta Suárez
and 2 others

Subjects

Microbiology

Topics
antimicrobial resistance antibiotics early life colonization intestinal microbiota consequences microbiome antibiotic resistance physiology neonate antibiotic prophylaxis prevalence children 16s rrna fecal microbiota intrapartum antimicrobial prophylaxis group b streptococcus health fatty acids establishment infant gut microbiota gut microbiota

Impact of intrapartum antimicrobial prophylaxis upon the intestinal microbiota and the prevalence of antibiotic resistance genes in vaginally delivered full-term neonates. (2017)

Abstract Disturbances in the early establishment of the intestinal microbiota may produce important implications for the infant's health and for the risk of disease later on. Different perinatal conditions may be affecting the development of the gut microbiota. Some of them, such as delivery mode or feeding habits, have been extensively assessed whereas others remain to be studied, being critical to identify their impact on the microbiota and, if any, to minimize it. Antibiotics are among the drugs most frequently used in early life, the use of intrapartum antimicrobial prophylaxis (IAP), present in over 30% of deliveries, being the most frequent source of exposure. However, our knowledge on the effects of IAP on the microbiota establishment is still limited. The aim of the present work was to evaluate the impact of IAP investigating a cohort of 40 full-term vaginally delivered infants born after an uncomplicated pregnancy, 18 of which were born from mothers receiving IAP. Fecal samples were collected at 2, 10, 30, and 90 days of age. We analyzed the composition of the fecal microbiota during the first 3 months of life by 16S rRNA gene sequencing and quantified fecal short chain fatty acids by gas chromatography. The presence of genes for resistance to antibiotics was determined by PCR in the samples from 1-month-old infants. Our results showed an altered pattern of intestinal microbiota establishment in IAP infants during the first weeks of life, with lower relative proportions of Actinobacteria and Bacteroidetes and increased of Preoteobacteria and Firmicutes. A delay in the increase on the levels of acetate was observed in IAP infants. The analyses of specific antibiotic resistance genes showed a higher occurrence of some β-lactamase coding genes in infants whose mothers received IAP. Our results indicate an effect of IAP on the establishing early microbiota during the first months of life, which represent a key moment for the development of the microbiota-induced host homeostasis. Understanding the impact of IAP in the gut microbiota development is essential for developing treatments to minimize it, favoring a proper gut microbiota development in IAP-exposed neonates.
Collections Ireland -> Teagasc -> PubMed
Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Research Institutes and Centres

Full list of authors on original publication

Miguel Gueimonde, Marco Ventura, Clara G de Los Reyes-Gavilán, Ana M Hernández-Barranco, Lidia Alaez, Nuria Fernández, Gonzalo Solís, Silvia Arboleya, Christian Milani, Marta Suárez and 2 others

Experts in our system

1
Miguel Gueimonde
Teagasc
Total Publications: 6
 
2
Marco Ventura
University College Cork
Total Publications: 31
 
4
Nuria Fernández
Teagasc
Total Publications: 3
 
5
Gonzalo Solís
Teagasc
Total Publications: 3
 
6
Silvia Arboleya
Teagasc
Total Publications: 13
 
7
Christian Milani
University College Cork
Total Publications: 9
 
8
Marta Suárez
Teagasc
Total Publications: 3