Type

Journal Article

Authors

B J Plant
C Stanton
R P Ross
M C Rea
F Shanahan
J A Eustace
C Shortt
M McCarthy
C Fleming
E T Flanagan
and 7 others

Subjects

Chemistry

Topics
adults gas chromatography mass spectrometry stable gut microbiota cystic fibrosis aromatic compounds fatty acid functionality

A pilot study demonstrating the altered gut microbiota functionality in stable adults with Cystic Fibrosis. (2017)

Abstract Cystic Fibrosis (CF) and its treatment result in an altered gut microbiota composition compared to non-CF controls. However, the impact of this on gut microbiota functionality has not been extensively characterised. Our aim was to conduct a proof-of-principle study to investigate if measurable changes in gut microbiota functionality occur in adult CF patients compared to controls. Metagenomic DNA was extracted from faecal samples from six CF patients and six non-CF controls and shotgun metagenomic sequencing was performed on the MiSeq platform. Metabolomic analysis using gas chromatography-mass spectrometry was conducted on faecal water. The gut microbiota of the CF group was significantly different compared to the non-CF controls, with significantly increased Firmicutes and decreased Bacteroidetes. Functionality was altered, with higher pathway abundances and gene families involved in lipid (e.g. PWY 6284 unsaturated fatty acid biosynthesis (p = 0.016)) and xenobiotic metabolism (e.g. PWY-5430 meta-cleavage pathway of aromatic compounds (p = 0.004)) in CF patients compared to the controls. Significant differences in metabolites occurred between the two groups. This proof-of-principle study demonstrates that measurable changes in gut microbiota functionality occur in CF patients compared to controls. Larger studies are thus needed to interrogate this further.
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Full list of authors on original publication

B J Plant, C Stanton, R P Ross, M C Rea, F Shanahan, J A Eustace, C Shortt, M McCarthy, C Fleming, E T Flanagan and 7 others

Experts in our system

1
Barry J Plant
University College Cork
Total Publications: 45
 
2
Catherine Stanton
Teagasc
Total Publications: 314
 
3
R Paul Ross
Teagasc
Total Publications: 499
 
4
Mary C Rea
Teagasc
Total Publications: 87
 
5
Fergus Shanahan
University College Cork
Total Publications: 259
 
6
Joseph A Eustace
University College Cork
Total Publications: 36
 
7
C Shortt
Teagasc
Total Publications: 4
 
8
Mary McCarthy
University College Cork
Total Publications: 42
 
9
C Fleming
Teagasc
Total Publications: 3