Type

Journal Article

Authors

John McKillen
Michael D Welsh
Gordon Allan
Joseph Cassidy
Bryan Markey
Bernadette Earley
Bernd Hoffmann
Michael J McMenamy
Catherine Mulholland

Subjects

Microbiology

Topics
real time pcr non viral reverse transcriptase polymerase chain reaction real time polymerase chain reaction technology transcription pcr detection bluetongue virus

The development of a real-time reverse transcription-polymerase chain reaction (rRT-PCR) assay using TaqMan technology for the pan detection of bluetongue virus (BTV). (2016)

Abstract Bluetongue virus (BTV) is an infectious, non-contagious viral disease of domestic and wild ruminants that is transmitted by adult females of certain Culicoides species. Since 2006, several serotypes including BTV-1, 2, 4, 6, 8, 9 and 16, have spread from the Mediterranean basin into Northern Europe for the first time. BTV-8 in particular, caused a major epidemic in northern Europe. As a result, it is evident that most European countries are at risk of BTV infection. The objective of this study was to develop and validate a real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) assay based on TaqMan technology for the detection of representative strains of all BTV serotypes. Primers and probes were based on genome segment 10 of the virus, the NS3 gene. The assay was tested for sensitivity, and specificity. The analytical sensitivity of the rRT-PCR assay was 200 copies of RNA per reaction. The assay did not amplify the closely related orbivirus epizootic hemorrhagic disease virus (EHDV) but successfully detected all BTV reference strains including clinical samples from animals experimentally infected with BTV-8. This real time RT-PCR assay offers a sensitive, specific and rapid alternative assay for the pan detection of BTV that could be used as part of a panel of diagnostic assays for the detection of all serotypes of BTV.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

John McKillen, Michael D Welsh, Gordon Allan, Joseph Cassidy, Bryan Markey, Bernadette Earley, Bernd Hoffmann, Michael J McMenamy, Catherine Mulholland

Experts in our system

1
Michael D Welsh
Teagasc
Total Publications: 11
 
2
Joseph P. Cassidy
University College Dublin
Total Publications: 55
 
3
B K Markey
University College Dublin
Total Publications: 43
 
4
Bernadette Earley
Teagasc
Total Publications: 120